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Uses of DNA profiling with PCR and Electrophoresis

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Introduction

USES OF DNA PROFILING WITH ELECTROPHORESIS AND PCR Genetic fingerprinting, DNA testing, DNA typing, and DNA profiling are techniques used to distinguish between individuals of the same species using only samples of their DNA. Statistically, no two people will have the same number of STR (short tandem repeats). When the DNA profile of a child is compared to the profiles of its genetic parents, it is possible to match one line in each STR area with a line in that area of the mother's profile. In this way, DNA profiling can also reveal non paternity. ...read more.

Middle

(2) DNA samples are placed into the holes at one end of the gel. (3) The electrical current is connected, with the negative current closest to the wells. The DNA samples are repelled by the negative charge. (4) The short strands move faster through the gel so they will be farther from the start. Strands of the same lengths end up together in groups that are visible to the naked eye. (5) The lengths are measured in bp (base pairs), which is not an exact measurement but accurate enough for the comparisons. ...read more.

Conclusion

Each time PCR is done, the quantity doubles. A limitation of PCR is that the DNA sample must be pure, otherwise any contamination or abnormalities will also be magnified. One case study of DNA profiling used for determining paternity was in the situation of Anna Nicole Smith's daughter, Dannielynn. Danielynn's paternity was in question ever since her birth in the Bahamas last September. After Anna Nicole Smith's death in February, the paternity of Dannielynn became all the more important, especially in the case of custody. After a many judges and trials, the testing was granted, and a sample was obtained from Dannielynn. The judge in the Bahamas ruled that the DNA test results confirmed at 99.99% certainty that a previous boyfriend and photojournalist was the father. ...read more.

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