Ultraviolet visible spectrophotometry. To measure different concentration (one or two substances) of solution, spectrophotometry can determinate an unknown solution.

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Ultraviolet - visible spectrophotometry

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Aim

UV - visible spectrophotometry can determinate the substance of solution. Different solution has different spectrum. To measure different concentration (one or two substances) of solution, spectrophotometry can determinate an unknown solution.

To grasp how a UV spectrophotometer works, understand the basic principles behind light waves.

Theory

UV - visible spectrophotometry (UV-Vis or UV/Vis) has a long history, which is the most widely used optical analysis. The ultraviolet region (about 450-200 nm) is particularly important for the qualitative and quantitative determination of many organic compounds. UV - visible spectrophotometry is based on the absorption or reflectance in the visible range directly affects the perceived color of the chemicals involved. Modern spectrophotometers draw this automatically. The measured spectrum is continuous, due to the fact that the different vibration and rotation states of the molecules make the absorption band wider.

The role of light in the material is that can produce different degrees of light absorption. Light is absorbed, since the form of heat energy is usually released, this energy is very small, usually imperceptible. We can measure the substances on the absorption