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University Degree: H.G. Wells

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  1. Enzyme-Linked Immunosorbent Assay or ELISA as it is more commonly known is an enzyme immunoassay (EIA), which is one of the most widely used applications of monoclonal antibodies.

    There are many variable techniques for ELISA, indirect, sandwich, and competitive are described below: An indirect EIA, the method that was used for the experiment carried out, can be used to detect specific antibodies in an antibody mixture, for example. This technique can be used to check if an individual is infected with HIV or is exposed to tuberculosis by testing their serum for presence of antibodies. In this case the desired antigen is attached to the well surface, and is carried out as follows: A specific primary antibody (or antibody mixture)

    • Word count: 2331

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