Determining vitamin C content in Orange Juice

Authors Avatar by rachael0693 (student)

Experiment #1 Determination of Vitamin C in Orange Juice

Date experiment preformed September 10, 2012

Date lab submitted Sept 18, 2012

Rachael Horne, student #120892960

Partners name: Geoff Williams

Lab section 3

IA: Parvina Shodieva

Purpose: The purpose of this lab was to determine the vitamin c content in varies orange juices.

Procedure:

For procedure reference the lab manual (CH110 Lab manual, fall 2012) pages 20-21

“Wilfred Laurier university department. Fall 2012. Determination of Vitamin C in orange Juice. Pages 20-21 in chemistry 110 lab manual. Wilfred Laurier University, ON, Canada.”

Results

 Table 1.

Qualitative observations of sample orange juices

Table 2.

 Results of titration of orange juice and iodine

Table 3.

Raw experimental data derived from titration of orange juice and iodine & calculated data resulting from experiment.

 Questions

C₆H₈O₆+ I₂  2Iˉ + C6H6O6

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Moles of iodine=moles of ascorbic acid. Moles of iodine where determined by n=c/v with known concentration of 0.0037M and experimental volume found during the titration.

Ex n(I₂)=0.0037M/0.0177L n=6.54x10^-5

 n(I₂)=n(C₆H₈O₆) knowing the moles of ascorbic acid and the molar mass we can now find the mass of C₆H₈O₆ in the sample using this equation

g=nxmm

Ex (6.54x10^-5mol)x(176.14g/mol)=(1.15x10^-2)g

1g=1000mg so 1.15x10^-2g=11.5mg

We do this for each trial to find the mg of ascorbic acid in each sample then average them using an avg formula  (a₁+a₂+a₃+a₄)/number of entries (in this case 4)

Ex

(11.5mg+8.62mg+3.51mg+4.72mg)/4=7.10mg

Therefore there is 7.10mg of ascorbic acid in the sample of 25ml of Tropicana

See all class values in appendix.

          Table 4.

    The mean and standard deviation using class data

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Table 5.

Mass of ascorbic acid in serving of sample orange juices and corresponding Daily % values

(Using class average data)

Table 6.       

Mass of ascorbic acid in serving of sample orange juice &corresponding daily % values

(Using my data)

Table 7.

Cost per litre of sampled orange juices

Table 8.

Calories and sugar content of varies samples of orange juice

Based on lab data, cost per litre, and nutritional information Tropicana Pasteurized “100% pure squeezed Orange Juice” is the best choice. This is because based on the results found in Table 8 ...

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