The Use Of Enzymes in Medicine
The Use Of Enzymes in Medicine The application of enzymes in medicine (or enzymology) is a constantly evolving industry. This is mainly due to huge improvements in technology (recombinant DNA) and genetic engineering in recent years (Fullick, 2000). Enzymes form a critical part of understanding diseases and their causes, as enzyme deficiencies are often central to many genetic diseases. Some harmful bacteria are also more effective due to their enzyme activity (www.enzymes.co.uk /answer_medicine.htm). Medically used enzymes can be used to diagnose, treat and cure many medical problems or dysfunctions. Enzymes are a hugely important part of our own metabolic pathways and biological processes, but can also be used in an industrial format. Often referred to as organic catalysts, they allow metabolic reactions to occur and control these reactions in such a way that the amount of products produced can comfortably meet the needs of the cells. Enzymes are specific to certain biochemical reactions. The first application of enzymes in medicine I am going to examine is Analysis. Glucose oxidase and peroxidase are the most frequently used enzymes for analysis. These two enzymes are immobilised (entrapped in an inert insoluble matrix in the process of immobilisation) onto a cellulose fibre pad. These pads forms the basis of Clinistix and Diastix. Glucose analysis (biosensors) allows
The use of pectinase in fruit juice production
The use of pectinase in fruit juice production I predict that there will be no juice produced to the apple sauce that had no enzyme and juice will produce to the apple sauce that had pectinase. A control is carried out in order to compare the rate of reaction between with pectinase and without pectin's. Pectin --> sugars + galataronic acid Pectin is a substance which helps to hold pant cell walls together. As a fruit ripens the plant produces proteolytic enzymes, which convert the insoluble protpectin of the unripe fruit into more soluble forms, causing the fruit to soften. When fruits are mashed and pressed to form juices these more soluble forms of pectin enter the juice, making it cloudy and causing the colour and flavour to deteriorate. Enzymes are specific in the reactions they catalyse, much more so than inorganic Catalysts. Normally, a given enzyme will Catalyse only one reaction, or type of reaction. The enzyme has an active site that helps it to recognise its substrate in a very specific way. Just like a key only fits into a specific lock, each enzyme has its own specific lock; each enzyme has its own specific substrate. This is called the lock and key theory. The enzymes never actually get consumed in the process; they just increase the rate of reactions. When enzymes denature the heat starts to destroy their shape and structure. The shape of the enzyme is so
The use of Recombinant DNA Technology can only Benefit Humans
The use of Recombinant DNA Technology can only Benefit Humans. The term gene technology refers to the process and techniques by which genes may be extracted from the DNA of one organism and inserted into the DNA of another organism, the host organism. In this way, the gene set of the host can be transformed, genetically modified. The modified DNA is called recombinant DNA because it has been re-combined to make a different set of codes. Recombinant DNA procedures involve splicing one piece of DNA into another. The newly formed composite is often grown up in a large amounts (or cloned in bacteria. Recombinant DNA and genetic technology are defined as Biotechnology, which is the use of a living organism to make a product or run a process. Recombinant DNA technology was first used commercially to produce human insulin from bacteria. In 1982, genetically engineered insulin was approved for use by diabetics. To provide a reliable source of human insulin, researchers obtained from the human cells of DNA carrying the gene with information for making human insulin. Researchers made a copy of DNA carrying this insulin gene and moved it into a bacterium. When the bacterium was grown in the lab, the microbe split from one cell into two cells, and both cells got a copy of the insulin gene. Some human
The use of recombinant DNA technology can only benefit humans
The use of recombinant DNA technology can only benefit humans Recombinant DNA technology is the technology of preparing recombinant DNA in vitro by cutting up DNA molecules and splicing together fragments from more than one organism.(1) This is the process of using recombinant DNA technology to enable the rapid production of human protein from a single gene of insulin. Firstly the single gene required must be isolated. This can be done three ways: Either by working backwards from the protein- Finding the amino acid sequence for the protein needed, the order of bases can be established using known genetic code. New DNA can be made from this sequence of bases resulting in artificial gene made from complementary DNA. By using Messenger RNA- mRNA molecules carrying the code for insulin are common in the cytoplasm of insulin. Or using DNA probes to find the gene required-A probe is a short single strand of DNA carrying the known genetic code we are looking for. So the location of the DNA probe is known, it is labelled with a radioactive fluorescent marker. The aim is for the probe to attach to its complementary base sequence within DNA extracted from human cells. Secondly the gene has to be cut from its DNA chain. Controlling this process are many restriction endonucleases (restriction enzymes). Each of these enzymes cut DNA at a different base sequence called a recognition
To investigate the effect of changing concentration of hydrogen peroxide (H2O2) on the enzyme catalase
To investigate the effect of changing concentration of hydrogen peroxide (H2O2) on the enzyme catalase 2 H2O2 + catalase 2H2O + O2 Background information H2O2 is a by-product of a chemical reaction inside the body metabolism. H2O2 has to be broken down into less toxic compounds or molecules. All enzymes are proteins. Temperature affects the rate an enzyme works, all enzymes work best at body temperature. Excess heat denatures enzymes rendering them useless. Catalase is an enzyme designed especially for H2O2. It works under a lock and key theory, where the catalase is the key and the H2O2 is the lock e.g. Rate Rate Substrate concentration enzyme concentration Reaches a plateau because all the Plenty of enzyme molecules to enzyme molecules are used up so deal with the substrate. substrate if you add more H2O2 the rate is is constant unchanged Preliminary work For my preliminary work we found three catalase sources and tested them to find the best one for our experiment. The three sources were yeast, apple and liver. We found that yeast was the fastest and that it released the most oxygen. We also found that the best amount
To investigate the effect of temperature on the enzyme catalase.
Aim: To investigate the effect of temperature on the enzyme catalase. Prediction: Using my existing scientific knowledge, I predict that as I raise the temperature to 30, 35, and 40, this is where we will see the greatest reaction. I predict this because enzymes are designed to react best at the body temperatures of the animals to which they belong. For a mammal, this is around 35-36. Catalysts are used to speed up biochemical reactions in the body. An enzyme is a protein molecule that speeds up chemical reactions in all living things. Without enzymes, these reactions would occur too slowly or not at all, and no life would be possible. All living cells make enzymes, but enzymes are not alive. Enzyme molecules function by altering other molecules. Enzymes combine with the altered molecules to form a complex molecular structure in which chemical reactions take place. The enzyme, which remains unchanged, then separates from the product of the reaction. Therefore, an enzyme is a sort of biological catalyst. Those enzymes identified now number more than 700. Enzymes are classified into several broad categories, such as hydrolytic, oxidising, and reducing, depending on the type of reaction they control. Hydrolytic enzymes accelerate reactions in which a substance is broken down into simpler compounds through reaction with water molecules. Oxidising enzymes, known as oxidises,
To investigate the effect of temperature on the enzyme catalase.
To investigate the effect of temperature on the enzyme catalase Aim: To investigate the effect of temperature on the enzyme catalase Apparatus Celery extract (catalase) Labels 20 Volume hydrogen peroxide Distilled water 6 100cm beakers 20cm and 50 cm measuring cylinders Balance Stopwatch Trough Clamps & stand 50cm conical flask + bung + tubing + 3 way tap 0cm syringe Beehive shelf Gas syringe Goggles Water bath at 25 & 35°C 250cm beakers & thermometer for water bath Method .Weigh out 1 sample of celery extract of 10g in a conical flask. 2.Place in a water bath at 25°C. 3.Place about 20cm hydrogen peroxide solution in a container in the same water bath. 4.Leave for 10 min to equilibrate. 5.Set up the apparatus as follows: -Fill a trough with water -Fill a measuring cylinder with water and invert in the trough (ensure that no air bubbles are in the water) -Secure the cylinder with a clamp & stand -Position the bung and 3 ways tap to fit on a conical flask, ensure that this can be held in place with a clamp & stand -Put the end of the tubing in the measuring cylinder 6.Make sure that the tap is closed and fill a syringe with 10cm hydrogen peroxide from the water bath. 7.Take the first conical flask containing the celery extract; arrange the apparatus as above and empty the syringe into the flask closing the 3-way tap start the stopwatch.
Transport across plasma membranes.
Transport across plasma membranes The cell surface membrane is approximately 7.5mm thick and is a bi-molecular phospholipid bilayer with inwardly directed hydrophobic (substances which repel water molecules) tails. It is also a fluid structure. A partially permeable membrane is one which allows some substances through but not others. There are a number of different ways in which substances are transported across plasma membranes. The first being diffusion, which occurs across the cell surface membrane. This is a passive process (requires no energy) by which substances move from a region of high concentration to a region of low concentration, of the same substance. The rate of diffusion depends on a number of factors: * The concentration gradient * The distance between the areas * The size of the molecules that are diffusing Particles of gas or solute can also diffuse through a membrane, as long as the membrane has pores that are larger than the particles. Every substance diffuses down its own concentration gradient. The concentration gradient of one substance has no effect on the concentration gradient of another substance. An example of diffusion is a tea bag in water - the flavour and colour from the tea inside the bag diffuse through the water. Another example is oxygen diffusing into a red blood cell in the body. Another process by which substances are transported
The Uses of Enzymes In Industry, Medicine and Analytical and Diagnostic Processes.
The Uses of Enzymes In Industry, Medicine and Analytical and Diagnostic Processes Enzymes are very precise protein molecules with a high specificity which are used to catalyse chemical reactions by lowering the activation energy required for the reaction to take place. It is these properties of being able to break down substances easily and bind specifically to certain chemicals that make enzymes very useful in many industries and practices throughout the world. In addition to this enzymes are not used up in experiments so products of processes are not contaminated with enzyme which could be a problem. This essay explains 3 uses of enzymes, in industry and food, diagnosing and analysing, and treating disease, explaining the function and advantages of enzyme use in each example. In industry enzymes are used for many processes such as brewing beer, baking bread, using pectinase to increase juice in fruit juice drinks and protease enzymes in washing detergents. Another example of the use of enzymes in industry is animal feeding. Many monogastric species such as poultry are fed food with enzymes added which break down substances in the food which the species body cannot digest. Many foodstuffs for farm animals such as wheat, rye, barley and oats contain non-starch polysaccharides (NSPs) which are an example of ANFs (anti-nutritional factors). They cannot be broken down by the
The ways in which organisms use ATP
The ways in which organisms use ATP The major energy currency molecule of the cell, ATP, is evaluated in the context of creationism. This complex molecule is critical for all life from the simplest to the most complex. It is only one of millions of enormously intricate nanomachines that needs to have been designed in order for life to exist on earth. This molecule is an excellent example of irreducible complexity because it is necessary in its entirety in order for even the simplest form of life to survive. The ATP is used for many cell functions including transport work moving substances across cell membranes. It is also used for mechanical work, supplying the energy needed for muscle contraction. It supplies energy not only to heart muscle (for blood circulation) and skeletal muscle (such as for gross body movement), but also to the chromosomes and flagella to enable them to carry out their many functions. A major role of ATP is in chemical work, supplying the needed energy to synthesize the multi-thousands of types of macromolecules that the cell needs to exist. ATP is also used as an on-off switch both to control chemical reactions and to send messages. The shape of the protein chains that produce the building blocks and other structures used in life is mostly determined by weak chemical bonds that are easily broken and remade. These chains can shorten, lengthen, and
