Investigate a factor which can affect the process of osmosis in a living plant tissue

Meetal Odedra 11V Biology coursework Investigate a factor which can affect the process of osmosis in a living plant tissue Planning For this coursework investigation I will be investigating factors that affect the process of osmosis in a living plant cell, but mainly focusing on one factor I have chosen. In order to do so, I have conducted some background research on osmosis to help me understand the investigation in hand. Background Scientific Research (Using D.G. Mackean's GCSE Biology textbook) Osmosis is defined as the movement of water molecules from a high concentration of water (potential) to a lower concentration of water (potential) down a concentration gradient, through a partially permeable membrane. When a substance such as sugar dissolves in water, it reduces the concentration of the water molecules because the sugar molecules attract some of the water molecules and stop them moving freely. Osmosis can be explained with the help of the diagram below: The diagram shows that the sugar molecules on the right have attracted half of the water molecules. There are now more free water molecules on the left than on the right so water molecules will diffuse more rapidly from left to right across the partially permeable membrane, than from right to left. This is because the net movement of the water molecules move from the high concentration of water

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Mr Chips: Investigation to find an isotonic solution for potatoes

Mr Chips: Investigation to find an isotonic solution for potatoes Introduction Investigation aimed to find out the amount of grams of salt solute needed to create an isotonic solution to prevent osmosis in fresh cut potato chips. Osmosis is the movement of water molecules through a selectively-permeable membrane down a water potential gradient. More specifically, it is the movement of water across a selectively permeable membrane from an area of high water potential (low solute concentration) to an area of low water potential (high solute concentration). The water molecules will continue to move through the semi-permeable membrane until both sides have reached a state of equilibrium. Isotonic solution is a solution in which its solute concentration is the same as the solute concentration of another solution with which it is compared In plant cells when water moves into the vacuole it increases in size and pushes the cell membrane against the cell wall, this causes the cellulose cell wall to stretch slightly and when it can stretch no further it becomes taut and firm. The pressure inside the cell rises and eventually the internal pressure of the cell is so high that no more water can enter the cell. This liquid or hydrostatic pressure works against osmosis. At this point the cell wall prevents the cell from bursting and is said to be fully turgid. Turgidity is very

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The effect of temperature on the hydrolysis of starch using amylase extracted from barley.

Sasha Caddy, RM11. 20/01/04 The effect of temperature on the hydrolysis of starch using amylase extracted from barley Interpretation of results: Enzymes are a class of proteins that catalyse chemical reactions, which increases the rate of a metabolic reaction. Most enzymes are specific, working on a particular or class of reactions. In this case I am using an enzyme known as amylase (a group of enzymes which convert starch to sugar), which is an important metabolic enzyme. Amylase is found in various parts of the body including the saliva of the parotid gland and the pancreas, e.g. ptyalin, which aids in the digestion of carbohydrates by speeding up specific digestive processes taking place from the mouth to the small intestines. However, in this experiment we are using amylase which has been extracted from barley. The function of amylase is to catalyze (to modify the rate of a chemical reaction by catalysis) the hydrolysis (decomposition of a chemical compound by reaction with water) of starch into glucose. Starch is a mixture of two compounds; amylose and amylopectin, both of these molecules are polymers which contain a large, variable number of a-glucose molecules linked to each other by condensation. Amylase acts on starch, which is a polysaccharide (a class of carbohydrates; starch, consisting of a number of twenty-five monosaccharides) and breaks it down into

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effect of concentration of copper sulphate on the action of amylase to break down starch.

Biology Coursework Abstract In this investigation I am going to explore the effect of concentration of copper sulphate on the action of amylase to break down starch. Copper sulphate is a very common chemical that can be used in a lot of different areas, but little has been discussed on its effect on amylase. I will find out which type of inhibitor copper ion is and how does it actually inhibits amylase, and also how concentration of copper sulpahte will affect the rate at which amylase works. Aim of investigation The aim of this investigation is to find out how concentration of copper sulphate affects the action of amylase. Hypothesis The higher the concentration of copper sulphate, the slower the amylase works. Rationale There are two ways that we could have consumed copper sulphate. Water pipes are responsible for transporting water, and some of them are made out of copper. Copper pipes can become rusted if there is high sulphate content in the water, basic copper sulphate is precipitated which can grow through the pipe wall creating pit holes [1]. Copper sulphate is also used in some places to treat sewer lines, tree roots are constantly looking for water and organic sources and sewer lines are the perfect site for them. Tree roots will penetrate and damage the pipes which will is expensive to repair. Copper sulfate kills tree roots without killing the tree or

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The Importance and Biological Functions of Carbohydrates.

The Importance and Biological Functions of Carbohydrates. Carbohydrates have many functions. This essay will look at some of them and also what carbohydrates are constructed of. A Carbohydrate molecule contains Carbon, Hydrogen and Oxygen. There are twice as many Hydrogens as there are Oxygens, the same proportion as water. Carbohydrates have the general formula of C (H O) Carbohydrates can be divided into three main types. These are monosaccharides (single sugar units), disaccharides (two sugar units) and polysaccharides (many sugar units). Different monosaccharides contain different numbers of carbon atoms. Trioses contain three, pentoses contain five and hexoses six. Carbohydrates have many different functions and come in many different forms. Ribose and Deoxyribose are both pentose monosaccharides and are found in RNA and DNA. Glucose and Fructose are both hexose monosaccharides. Glucose is an important source of energy in respiration and Fructose is found in fruits. Sucrose is a disaccharide formed from Glucose and fructose. It is the form in which carbohydrates are transported in plants. Maltose is a disaccharide of glucose and is formed from the digestion of starch. The carbohydrate in milk is lactose and it is formed from Glucose and galactose. Important polysaccharides include Starch, Glycogen and Cellulose. They are all made up from Glucose but have different

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Following the Progress of an Enzyme Controlled Reaction

Following the Progress of an Enzyme Controlled Reaction Plan Enzymes are a widely used source of biological catalyst; they are used in widely in industry as in the biological aspects. Enzymes are biological catalyst; this means that they will speed up a reaction with out becoming used up. The enzymes for this by not actually interfering with the reaction its self but basically align the two substrates on the active site of the enzyme. Amylases are widely spread enzymes that hydrolyse starch to maltose. They are often found in two forms, a-amylase, which degrades starch molecules into, fragments 10 glucose residues long and b-amylase, which breaks down these into maltose, made up of two glucose molecules. Both work by hydrolysis adding one molecule of water across glycosidic link. Hypothesis My hypothesis is that as the time of the enzyme reaction goes on the amount of substrate reacted by time goes in a proportional relationship. In theory time Vs substrate concentration should have a proportional relationship as the relative enzymes will all have plenty of the substrate molecules to align with, therefore creating a constant time for the reaction. The substrate being used is starch, starch is widely found inn various substances. It is greatly found in bread, the starch is a very useful nutrient for human life forms as it is the source for sugars, which produce energy for

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The effect of drugs on the nervous system

The effect of drugs on the nervous system A drug is defined as a substance that, when absorbed into the body, alters a normal bodily function. Some are able to do this, as they are capable of producing an array of different effects on the nervous system. The reason why affecting the nervous system of an organism is potentially so significant is due to the nature of it. The fact the nervous system directs the functions of all the tissues of the body demonstrates its considerable role within the body. It can therefore be assumed that taking substances which affect how it works may lead to a distortion in the way your body is run, producing unsafe side effects. The term nervous system is actually used to describe two divisions. The central nervous system (CNS) is the part which contains the brain and spinal cord. The peripheral nervous system (PNS) however, consists of all the sensory neurones used to detect stimuli. The PNS receives thousands of sensory inputs and transmits them to the brain via the spinal cord. The brain will then process this information, discarding around 99% of it as unimportant. After this sensory information has been processed, areas of the nervous system generate nerve impulses to organs or tissue and form a suitable response. As influences from chemicals are able to affect how the nervous system functions, it can be assumed that chemicals such as

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Affect Of Varying Salt Concentration on Red Blood Cell Haemolysis

COURSEWORK INVESTIGATION Affect Of Varying Salt Concentration on Red Blood Cell Haemolysis Tahir Aziz CONTENTS > Plan > Outline method > Key variables > Risk assessment > Preliminary results > Method > Results of control experiments > Results > Conclusions > Main trends and patterns > Explanation of results > Experimental limitations Affect Of Varying Salt Concentration on Red Blood Cell Haemolysis Abstract The average adult has about five litres of blood living inside of their body, coursing through their vessels, delivering essential elements, and removing harmful wastes. Without blood, the human body would stop working. Blood is the fluid of life, transporting oxygen from the lungs to body tissue and carbon dioxide from body tissue to the lungs. Blood is the fluid of growth, transporting nourishment from digestion and hormones from glands throughout the body. Blood is the fluid of health, transporting disease fighting substances to the tissue and waste to the kidneys. Because it contains living cells, blood is alive. Red blood cells and white blood cells are responsible for nourishing and cleansing the body. Since the cells are alive, they too need nourishment. Vitamins and Minerals keep the blood healthy. The blood cells have a definite life cycle, just as all living organisms do. Approximately 55 percent of blood is plasma, a straw-collared clear

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The Ways in Which Organisms Use ATP

The Ways in Which Organisms Use ATP ATP, the standard abbreviation of Adenosine-5'-triphosphate is a multifunctional nucleotide used in cells as a coenzyme, and can be best summarised as the standard energy currency universal to all organisms, and as such is utilised in metabolic (and other) processes throughout the cells of organisms, and is highly adapted to its function therein due to its high instability in aqueous solutions (eg tissue fluid) due to its easily hydrolysable phosphoannhydride bonds which when broken release a proportionally huge amount of energy. ATP is required during the contraction of skeletal muscle. ADP is released by the myosin head, this allows it to change shape thereby pulling the actin filament across itself. In order to detach the myosin molecule (for it to bind to a myosin binding site further along the actin) ATP binds to the myosin head, where it is hydrolysed to release ADP (which remains on the head) and inorganic phosphate. The energy released allows the myosin head to resume its normal position prior to the release of its ADP molecule, ready to bind further along the actin filament. Furthermore, ATP is utilised elsewhere within the same process, as it is used by carrier proteins on the membrane of the sarcoplasmic reticulum as a source of energy for the active transport of Ca2+ ions into the sarcoplasmic reticulum. It is when these

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Enzymes in agriculture.

Enzymes in Industry and Medicine by David Harder Enzymes in agriculture The only major agricultural area to utilise enzymes is the feeding of monogastric animals. There are two applications, which currently utilise enzymes. Biological silage inoculants frequently contain enzymes in addition to lactic acid bacteria. The enzymes in such products partially breakdown some of the cell wall components of the plant material to be ensiled into soluble sugars. These liberated sugars are then metabolised by the natural or applied lactic acid bacteria such as Lactobacilli or Pediococci into lactic acid, which reduces the pH and so ensiles the crop. Some enzyme preparations have been reported to improve the utilisation of feeds for ruminant animals. The use of enzymes in arable agriculture especially in the processing of some major crops and in waste disposal systems is areas, which has not been fully investigated. Enzymes in baking Bread baking is one of the most common food processing techniques throughout the world although the bread products of different countries vary in their finished form. The basic component of all bread is wheat flour to which is added water, salt and yeast. Other ingredients are sometimes added such as sugar, fats and flavouring components. The main components of wheat flour are starch, protein and fibre. The wheat flour has naturally occurring enzymes

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