Comparing High Pressure Liquid Chromatography and Gas Chromatography
Comparing High Pressure Liquid Chromatography and Gas Chromatography
HPLC and GC have different approaches to separating components in mixtures. HPLC deals with separating particularly non-volatile and liquid substances such as ions, polymers and other complex structured molecules into their components; whereas, GC deals with volatile and gaseous substances or the substances could be vaporised (organic or inorganic molecules such as alcohols) while they are in the injection unit.
In HPLC high pressures are given from a pump in order to force the mobile phase through the column and interact with the components within the mixture. This is because compounds that react very slowly due to their complex structure are being separated within the column. A vacuum degasser is also present in HPLC and it removes the air bubbles that are in the mobile phase; otherwise these air bubbles will give false peaks in the chromatogram.