SL Biology: Practical 5: Report
Aim: to investigate the Effect of Temperature on Membranes
Background: The colour of beetroot is due to the presence of a red pigment, betacyanin in the cell sap. As long as the cells and their membranes are intact, the betacyanine remains inside the vacuoles in the cells. However, if the membranes are stressed of damaged, betacyanin will leak through the membrane and produce a red colour in the water surrounding the beet tissue. Such stress or damage can be caused by heat. The intensity of this red colour will allow for the assessment of the damage produced by experimental treatment.
Hypothesis: The higher the temperature, the higher the damage done to the cell membrane, and thus the more betacyanin released into the water surrounding the beet tissue. This will lead to a more intense shade of red.
Materials, procedure: [as stated in practical]
Variables:
Dependent variables: the intensity of the redness in the water
Independent variables: the 6 temperatures of water which the samples were submerged in