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Experiment to demonstrate the action of amylase on starch solution.

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Experiment to demonstrate the action of amylase on starch solution Esme Power Plan Scientific knowledge: Substances called catalysts speed up many chemical reactions. Catalysts called enzymes control the metabolic reactions in the body. Amylase is an enzyme; it is present in the digestive systems of many animals. Amylase speeds up the breakdown of long chain starch molecules. I know that the more common enzymes work best at around 37.c as this is our natural body temperature. If this temperature begins to rise the reactions slow down, this will continue to happen until the enzyme is denatured. It becomes denatured at around 60.c, this happens because the enzyme becomes misshapen. This means that the enzyme will no longer work because it will no longer fit. When the two join then you get the chemical reaction. Preliminary work: I watched someone else do this experiment and saw how it was to be done. This helped me see how the experiment worked and how to do things correctly. I have studied enzymes so this has helped me understand what is happening. ...read more.


I will keep the same person to decide whether the starch has been broken down as everyone has different eyes and what we see is different. I could look at other students results in this field to confirm my results. I want to keep the starch solution, iodine and amylase solution constant through out the experiment, I will only change the temperature to make the experiment a fair test. Prediction- Using scientific knoledge I can predict that the enzyme will work best around 30.c - 40.c as this is around body temperature, and amylase works best around body temperature. At high or low temperatures I think that the amylase will work more slowly than at 30.c. At 70- 80.c I think that the enzyme will be denatured, we can tell this by the Iodine solution showing us that the starch has not broken down. Obtaining evidence. I have recorded my results in a table, then a graph. Temperature (.c) time took to break down starch Of starch solution minutes and seconds 1. ...read more.


My prediction was wrong as I said that the amylase would work best at 30- 40.c, where as my results show me that it works best at 50.c. I did predict correctly that the reaction would be slowest at either low or high temperature, and would denature around 70- 80.c. Evaluation. I found it difficult to keep the temperature of the starch constant when doing the experiment. If I did this experiment again I would keep the solution of starch and amylase in a water-bath of the right temperature. I think that because the temperature cooled down as time went by my experiment wasn't totally accurate. I decided that the experiment at 80.c did not fit the correct pattern, so I repeated it. The second time it gave me the results I predicted. The timing might not have been exactly accurate and this could have effected my results. If I did this experiment again I would want to use a water-bath for more accuracy in controlling the temperatures. Also I would have liked to repeat all of the experiment one more time, so that my average would be more precise, with 1 or 2 results, they can not be that reliable. ...read more.

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