• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

How enzyme concentration affects the rate of reaction

Extracts from this document...


How enzyme concentration affects the rate of reaction Aim- The aim of the experiment is to find the relationship between the concentration of protease (enzyme) and the rate at which the proteins breakdown. Using different concentrations of enzymes the time taken for the solution to go clear will be observed. Rate of reaction refers to the molecules and how often they collide with each other and with how much energy. The kinetic energy theory states that particles have to collide with each with enough energy in order to speed up a reaction. Enzyme activity can be dependent on a number of factors. Increasing the temperature increases the rate of reaction as particles gain more kinetic energy and speed up the reaction. The pH of a solution and surface area of a substance also speed up reactions. In this experiment it will be investigated how increasing the concentration can affect the rate of reaction. With all the factors that speed up a reaction there is an optimum point when the enzymes will denature. Enzymes are proteins that act as catalysts. They speed up chemical reactions which would otherwise take a very long time. The advantages of enzymes are that they do not get used up in the reaction but stay unchanged. So only a small amount of enzyme is needed as they can be reused. To understand the significance of enzymes in an enzyme-catalyzed reaction some information about how they work must be known. ...read more.


Using different sizes could affect the results because the solution may not be as much concentrated if using as small tube compared to a big one. The temperature of the water baths must be kept at 20c to ensure that this variable is controlled and that the results are accurate as much as possible Safety- * The first and foremost thing before the start of the experiment is to wash hands with warm water and soap. This will ensure that no unwanted substances are transferred during the experiment. * The workbench needs to be tidy and all stools tucked underneath the table so that there is no risk of tripping over or a spillage. If equipment is broken or if there is a spillage it should be cleaned up immediately as it could cause a hazard for someone else. * Loose pieces of clothing and hair will need to be tied back so that they do not come in the way whilst conducting the experiment. A laboratory coat, gloves and goggles must be worn as all times during the experiment to protect clothing and the body. The enzymes could irritate the body so by wearing gloves it will ensure that they do not come into direct contact with skin * Care must be taken that liquid does not come into contact with electrical equipment * Food and drink must not be consumed in the science laboratory especially when conducting the experiment Ethical issues-. ...read more.


Because no one persons eyesight is the same the results cannot be deemed accurate and valid however they would still be reliable. However the results cannot be guaranteed absolutely reliable, as the interpretation of when the solution goes clear could be different for all the tubes. To overcome this problem a colorimeter could be used to measure the light intensity. A colorimeter would ensure that no random errors are met. Also by making sure that the colorimeter is calibrated before each use would ensure that systematic errors are not made. Because only small concentrations were used in the experiment the time when the enzymes stopped working could not really be identified. Larger concentrations for example 10, 20, 30 could be used. This way the point at which the enzymes started to denature could have been identified. Care was not taken to ensure that other variables such as PH and temperatures were controlled. This could have affected the results and although the anomalous result is not far off it could be that the anomalous result is because one of the two variables was not controlled Further work- Only one type of enzyme was used therefore it cannot really be concluded if this is the general trend for all enzymes. Another investigation with different enzymes but using the same protein could be conducted to see if the general trend is the same. To ensure that the results would be accurate the same methods, apparatus and precautions would need to be taken. However the improvements and modifications mentioned would need to be taken into account ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month
  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work