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Investigating The Effect Of Temperature Change On The Permeability Of Beetroot Cells.

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Introduction

Investigating The Effect Of Temperature Change On The Permeability Of Beetroot Cells. Aim This investigation aims to determine how an increase in the surrounding temperature affects plasma membrane of a typical plant cell structure. Hypothesis Increasing the temperature above a certain extent will damage and denature the plasma membrane and cause the cytoplasm and other substances contained within the membrane to leak out. Introduction Beetroot has a red pigment (anthocyanin) stored within its cells; it is prevented from leaking out by its cell membrane. The membrane is an extremely thin layer (8 to 10 nanometers (nm)) thick which is partially permeable. As we know, cell membranes are made up of lipids and proteins, and proteins tend to be affected by temperature. This investigation intends upon finding out if temperature has any effects on the permeability of beetroot cells, my prediction is that it does. This is because when the temperature is increased, a number of bonds in the protein molecules of the cell membrane are weakened. ...read more.

Middle

* A Beaker * Thermometer * Bunsen Burner, Fireproof Mat, Gauze, stand * A Tile, Scalpel * Test tube rack * Pipette * Beetroot cores cut into discs of approx. 1mm thickness * Cuvettes (Colorimeter) * Measuring tube Plan 1) Cut the Beetroot cores into discs (1mm thickness approx). Try making sure as far as possible that they are all of the same diameter and thickness, so as to reduce any errors unrelated to the actual aim of the experiment. 2) The slicing of Beetroot into discs will cause cells to rupture and release the red pigment, these damaged cells are insignificant and hence make sure to wash the Beetroot discs out in cold-water until they no longer change colour and the water remains clear, thus no pigment is showing. 3) As the pilot investigation seems to indicate the temperature of leakage to be between 40o C - 50o C, set the water bath(s) using conventional apparatus such as Bunsen and tripod etc... ...read more.

Conclusion

9) Move the Cuvette changeover control (located on the left hand side panel) to the "ref " position and, using the coarse and fine sensitivity controls, set the metre to read 100% transmission (or 0% absorption). 10) Move the Cuvette change over control to the test position and read the percentage transmission or absorbance of the standard solution directly from the meter. 11) Make a note of the readings and then do the same for test tubes B-F. 12) After results have been collected, tabulate the results, draw graph and deduce any conclusions obtained from the results. As you can see from my plan, the dependent variable is the calorimeter measurements and the independent variable is the temperature control. Thus make sure all other variables such as volume of water in test tubes, size of discs and calorimeter settings are all kept constant, so as to reduce any inconsistent outcomes. ?? ?? ?? ?? Luqman Patel A/S Biology ...read more.

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Here's what a teacher thought of this essay


** Plan only included. This plan includes a fairly detailed method but is lacking in a number of key elements that would be expected for A level coursework.
To improve
The inclusion of background research beyond that found in most A level textbooks is expected to help inform the plan. References should be included.
The aim/ hypothesis needs to be clearly stated in terms of the dependent and independent variables.
There should be a full consideration of the key experimental variables and how they are to be controlled. The apparatus that is to be used should be justified as well as listed.
There is no attempt to assess safety and this is usually expected in newer mark schemes.
The method mentions a pilot experiment. If this has been carried out the results should be included and discussed.

Marked by teacher Stevie Fleming 26/07/2013

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