Lipids Analysis. Aim: To carry out a comparative analysis of the fat composition of two different types of milk,

Authors Avatar by JamminInJamica (student)

Date: October 2, 2013

Title: Fats

Aim: To carry out a comparative analysis of the fat composition of two different types of milk, S1 and S2.

Apparatus: Test tubes, measuring cylinder, copper (ii) sulphate solution, pipette, Sudan (ii), test tube corks , two milk samples, milk powder solution (S1) and cow’s milk (S2).

Method:

Part One:

        1 cm3 of copper (ii) sulphate was measured then place into a clean test tube. A little of S1 was sucked up using a clean pipette and gently released into the CuSO4 (aq), about 2cm below the surface. The pipette was withdrawn slowly from the solution and the movement of S1 was carefully observed as well as the time for the drop to reach the surface of the solution. This procedure was repeated using S2 and clean apparatus.

Part Two:

        0.3 cm3 of S1 and S2 was measured and placed into separate, dry and clean test tube. These test tubes were labeled. 5 drops of Sudan (iii) was added and the test tubes were then shaken. The tubes were filled with water (about ½ way), a cork was placed in each and then shaken vigorously several times. The contents were observed immediately after being shaken and after 10 minutes.

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        Both observations were recorded and the necessary comparisons were then made.

Precautions:

To maintain the accuracy certain precaution are taken. The drops need to be at same depth in the solution so they travel the same distance. All apparatus should be cleaned before dealing with different solutions. Adding the same amount of water into the test tubes in part two.

        

Observations:

Table 1 showing the observations in part one of the investigation.

 

Table 2 showing the observations in part two of the investigation.

Discussion:

A lot of lipids function as long-term energy storage. One ...

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