The equation for water potential is
Water potential of a cell = solute potential of ions inside cell + pressure potential of cell wall
Ψ cell = Ψ s + Ψ p
I got my research from the following sources
- Letts AS success – biology
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I found these sources very beneficial. They enabled me to grasp the basic concepts of osmosis, water potential and plasmolysis.
Variables
There are many variables that can affect the results of this experiment. To stop this from happening I will keep these factors the same.
- Size – if the size of the potato is different each time it will make my experiment unfair as there will not be the same amount of surface area each time meaning there is more potato cells to conduct osmosis
- pH – if the pH of the experiment is different there water molecules ability to conduct osmosis ill be affected
- Pressure – If the pressure is changed there is a chance the water potential gradient could alter which would change the rate of osmosis
- Time- if the time is not kept the same for each experiment the more weight the potato may gain or loose making the experiment inaccurate
There is however some factors which I will change. In the preliminary experiment I will change the time the potato is in the sugar solution and the length of potato I use so I can decide which factors ill be best for me to use in my main experiment. The only factor I will change in my main experiment is the concentration. By keeping everything else the same I will be able to see how the concentration affects the rate of osmosis without worrying my results are inaccurate due to other factors.
Prediction
I predict that as the concentration of sucrose increases the weight of the potato will decrease. This is because osmosis is the process of diffusion of water molecules from a stronger solution to a weaker solution through a semi permeable membrane. The pores in the potato cell means the water molecules can move in and out of the potato cell depending on the concentration gradient between the potato and sucrose solution. If there is a higher concentration of water in the sucrose solution than in the potato, the water will pass from the sucrose solution into the potato causing the potato to increase in weight. However if there is a higher water concentration in the potato then water will diffuse from the potato into the sucrose solution.
Equipment
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Potato – I will use the potato to put in a solution and measure the rate of osmosis. The type of potato ill be kept the same and the length to ensure it is a fair test.
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Test tubes – This is what I will use to put the solution and potato in and I will keep them in there for 15 minutes
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Beakers – I will use beakers to put distilled water in so I can wash the starchy layer of the potatoes as this could affect my experiment
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Potato cutter – I will use this to cut the potato. I will use the same size all through my experiment to ensure it is the same width.
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Knife – I will use a knife to cut the potato to length and also to cut the end of the potato so that there is no extra potato skin there to affect my experiment
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Sugar solution – I will put potato pieces in different concentrations of sugar solution these will range from 0% to 100%
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Scale – Scales accurate to 2d.p will be used to measure the weight both before and after the potato has been in the sugar solution. I will then work out the difference to see if the potato has gained weight or lost weight
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Stop watch – The stop watch will be used so I can time the 15 minutes the potato has to spend in the sugar solution
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Measuring cylinder – I will use a 2ml measuring cylinder so I can accurately measure different concentrations of the sugar solution
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Paper towels –I will use these to dry the potatoes before they are weighed
Preliminary
The aim of my preliminary is to see which are the most suitable time and length to use in my main experiment. I will do the experiment as stated and use my results to come up with the suitable measurements.
Results
From my results I have decided to use15 minutes to soak the potato in the sugar solution. I feel this is best as it produced consistent results and gave me enough time to set up the next experiment whilst the potato was in the solution. This makes my experiment efficient.
I also decided to use 3cm long pieces of potato. The results produced are consistent and are also big enough to spot a change in the weight of potato which will make it easier for me to analyse my results.
By doing the preliminary experiment I have come to the conclusion that the experiment I am using is an accurate ay of obtaining the results I want to. So I will not change anything for my main experiment.
Main experiment
The aim of my main experiment is to se if there is a link between the increasing concentration and the rate of osmosis. I will do this by following the method below. I will use a time limit of 15 minutes and a potato length of 3cm
Method
- Firstly, I will collect all the equipment I need to carry out the experiment
- After this I will use a potato cutter to cut 6 cylinder pieces of potato and then use a ruler and knife to cut them to the correct length, making sure to cut the ends off
- I will soak the potatoes in a beaker of distilled water for 1 minute to wash the starch layer off and dry them using paper towels. I will then record their weight using scales.
- Then I will fill 6 test tubes with the different concentrations of sugar solution and label them so I know which is which
- I’ll add one piece of potato into each of the 6 test tubes and time for 15 minutes using a stop watch
- Once the 15 minutes is up I will pat the potato dry, weigh them again and record the results
- Using my results I will work out if the potato has gained or lost weight
- I will redo the experiment another two times to erase any anomalies that may occur
Safety
As the solution I am working with is not a hazard to the skin it does not matter if contact is made with the skin. The only hazard in this experiment is using the knife to cut the potatoes to length. So I will exercise caution when using the knife.
Results
Test 1
Test 2
Test 3
Anomalies – These will not be included when working out the averages
Analysis
In my results I found a definite relationship between weight of a potato and concentration of the sugar solution. This is shown by the line of bets fit on my graph which produces a smooth curve sloping downwards. The relationship is as the concentration increases the weight of the potato decreases after being soaked in it for a certain length of time. This proves my prediction was right.
My prediction is supported by the evidence in the graph. My results generally follow the line of best fit and the line of best fit stays inside the error bar which means it is accurate. I did however have an anomaly in my results however I did not include this when calculating the averages so it should not affect my results. There could be any number of reasons for this anomaly.
Graph two of my results shows the average rate of osmosis in my experiments. The line of best fit is a straight line. There is an anomaly in this at 20%. This could be due to a measuring error. Other than at 20% my results follow a pattern
Conclusion
In conclusion it’s clear from my results that the amount of glucose in a solution can affect the rate of osmosis. This is because if there is a higher concentration of water in a potato the water will then move out of the potato into the sucrose solution faster. Which means the potato will gain weight faster. Also the higher the concentration of sucrose there will be a steeper water potential gradient meaning the process of osmosis happens faster.
Evaluation
Overall I think that my results are relatively accurate, this is because I repeated my experiment 3 times. The evidence for this is the results following the line of best fit. I did however have an anomalous result, although I did not include this when calculating the averages. This could be down to any number of reasons including an inaccuracy when measuring out the solution. It could also be possible that the amount of time sI patted each potato piece to dry it could affect the end result. Although I can no be accurate when saying what affected my experiment I can guess at some of the factors that may have affected it.
If I was to do the experiment again there would be a few things I would alter so I could increase the reliability of the results. Firstly I would put a lid on the test tubes. This is because water could evaporate out of the test tube causing the concentration to change. Another thing I would change is I would dry the potatoes in exactly the same way for the same amount of time for every experiment as a little bit of water left on the potato piece can affect its weight. The last thing I would change is the ruler I used. I would make sure the ruler had millimetres on it so I could make sure that each potato piece is exactly the same length.
I think the results my graph produced are very reliable. They helped me come to a valid conclusion which I had predicted.
To further my experiment I could vary the vegetables I use to see if the effect of concentration is the same on them. Examples of vegetables I could use are swede or parsnip, as these vegetables have similar properties to the potato.