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Semi quantative Benedict's test

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Semi quantative Benedict's test The Benedict's test is for testing the presence of simple sugars in sample if sugars are present a colour change will occur from blue to red. However although the benedict's test shows the presence of sugars for which it is useful it cannot be used to accurately determine the concentration of sugar in a sample solution. If you follow the method that we have followed which involved adding specific concentrations of glucose to the benedict's to use as a chart to estimate the glucose concentration of an unknown solution X. Although this gives you a rough estimate of the concentration it is very inaccurate. For example the mystery solution X was a pale orange colour which was in-between the colours in my first and second test tube therefore the glucose concentration of solution X could have been anything in between 1% and 10% glucose concentration. ...read more.


2) Secondly the time that all the test tubes were kept in the water bath had to be the same for all test tubes including solution X. This is because the colour may have developed more or less depending on the time left in the water bath. 3) Thirdly temperature in the water bath had to remain the same for all of the test tubes because this could also have affected the colours of the solutions hence affecting the results. The time in the bath and the temperature in the bath were very simple to keep constant because all test tubes were put in the same bath at the same time and taken out at the same time. Therefore all test tubes were exposed the same water bath conditions. ...read more.


2) THE TWO BEAKER WASH: The second method I learnt was the two beaker wash this is simply an efficient way of cleaning your syringe. It involves filling one beaker full of distilled water and leaving another empty then filling your syringe full of distilled water then emptying it into the beaker, this process should repeated 3 times for thorough cleaning. Although this is a simple technique it is very important to wash your syringe when changing between concentrations because your results can easily be influenced by residue of 'left over' from the previous concentration. 3) SELECTION OF SYRINGE: The third method I learnt was simply how to select the best syringe for the job. When selecting the size of your syringe you should always pick the smallest syringe that it is possible to do the job with on one pump of the syringe. This is to add accuracy. ?? ?? ?? ?? Jagpal Randhawa ...read more.

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