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The Breakdown of Starch by Amylase.

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The Breakdown of Starch by Amylase Plan: In this experiment I will be testing the amount of time it takes for the enzyme Amylase to break down Starch into Glucose at different temperatures. I will do this by heating the Starch and amylase solution, starting at 5?C and increasing it an extra 5?C up to 60?C after each test, taking a sample of it every minute, then adding it into a tray of iodine samples. To tell whether the Amylase has broken down the Starch into Glucose the colour of the solution will change when added to the Iodine. If the Starch is still present the solution will turn a black/dark blue colour. If the Starch has been broken down the solution will turn orange. The apparatus I will use to conduct my experiment will be as followed: * Waterbath - I will use this to heat the Starch and Amylase solution. ...read more.


I do this to examine the affects of heating the solution. The chemical reaction will take less time as you heat the solution, as the particles vibrate more, increasing the amount of collisions thus resulting in a quicker reaction. This theory is called the Particle Theory. Every minute of warming the solution, I will take a sample and use a pipette to add a sample to a tray of Iodine. If starch is present the colour of the samples will change to orange, if not the solutions will turn black/dark blue. To make this a fair test I will keep the amount of amylase and starch the same because if the amount of either substance was different the results would be inconclusive. I could vary the test conditions in many different ways: * Change the temperature - as due to the Particle Theory, the chemical reaction will increase in speed if heated. ...read more.


If the volume of Amylase was increased the reaction would on the other hand take less time to react as if there were more Starch, as the Enzymes would control more surface area. I would have to decide on suitable ranges of all the substances, temperatures and the time gap before each sample taken. I believe these to be suitable ranges: * Temperature of waterbath: 5?C and increase an extra 5?C up to 60?C. * I will use the same amount of Starch and Amylase in the solution, as if there were more of either substance the reaction times would increase. * I will take a sample of the heated substance every minute. So leaving time for reaction. I believe these would be suitable test condition because they are fair up to a point and would achieve the best results. Prediction: I think that as the temperature is increased the amount of time taken till breakdown will decrease, because of the effects of the particle theory. Sam Stiff ...read more.

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