The effect of enzyme concentration on the activity of amylase.

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The effect of enzyme concentration on the activity of amylase.

I am going to test in the effect of the concentration of the enzyme, the only thing that I should be changing in each experiment is the concentration of the enzyme and nothing else. This will make all of the tests identical (apart from the enzymes concentration) which means the experiment should be accurate and fair. Because of this any differences in the results of my experiment I will be able to put down to the concentration of the enzyme. If I was to use two variables, and there were a difference in the results, e.g. I changed the temperature as well as the concentration of the enzyme and the reaction sped up, I would not know which of the factors had done this and therefore could not reach a valid conclusion. The variables that have to be kept constant throughout all of the experiments are Temperature, surface area and pH.

Variables:

These factors could affect the rate of reaction on an enzyme:

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  • pH

  • Concentration

  • Temperature

  • Surface Area

pH - Enzymes function at different pH values. In neutral conditions the amount of oxygen gas given of in an enzyme-catalysed reaction will increase.

Concentration - In concentrated solution there are more collisions between each particle, so the reaction occurs more quickly.

Temperature - Reactions go faster as temperature rises. The rate of reaction also increases as the temperature rises, but with enzyme-catalysed reactions the reaction rate starts to decrease when the temperature is above 40 C. This is because enzymes are proteins and their ...

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