The reaction Between Gelatine and Trypsin.

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Dipak Vyas 11TW

Biology Coursework The reaction Between Gelatine and Trypsin

Aim

I am going to investigate how photographic film can be recycled to be reused again.

The photographic film has a silver compound on it which needs to be separated from

the plastic. If the silver compound was to come off then the plastic would go clear. If

we were to use an industrial process it would be too expensive, so our task is to make

it cost efficient. Exposed photographic film contains black grains of silver compound.

The particles of silver are attached to the film by an insoluble compound called

gelatine. To achieve this goal I have been asked to find the optimum temperature at

which this process is most efficient. I will use an enzyme called Trypsin to dissolve

the gelatine in the photographic film. However gelatine is in soluble in water if the

water is not at a high temperature. When the enzyme digests the gelatine it forms

soluble amino acids. These acids dissolve and take the silver compounds with them.

At the end the plastic should appear see-through.

Prediction

There are many important factors that will affect my experiment. The most important

factors will be concentration of the Trypsin, temperature of the Trypsin and surface

area of the film piece. I will be using temperature as a variable for this experiment;

this means that the other factors mentioned above will have to be controlled. I predict

that that as the temperature of the Trypsin increases the rate of the reaction will get

faster. Even when the enzyme has denatured the rate of reaction will still continue to

rise.

Reasons

Trypsin is a biological catalysts that speed up the rate of reaction without them

themselves being used up. The enzyme Trypsin is extra cellular; this means it is found

outside a cell. There are many reasons for the choices that I have made in my

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prediction. I have predicted the collation between the rate of reaction and the

temperature because of the collision theory. The collision theory is affected by the

temperature of the solution. If the temperature is high the chance of a successful

collision is increased. This is because the extra temperature supplies extra energy to

the particles. The particles must have a certain amount of activation energy to have a

successful collision. In this case the trypsin will collide with the gelatine and attach

itself to it. Trypsin must ...

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