• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

To investigate the effect of Trypsin using a photographic film

Extracts from this document...

Introduction

Aim: To investigate the effect of Trypsin using a photographic film Prediction: In this experiment there are two variables. Firstly, the pH. Changes in pH would alter or totally inhibit the enzyme from catalysing a reaction This change in the pH will affect the polar and non-polar intramolecular attractive and repulsive forces and alter the shape of the enzyme and the active site as well to the point where the substrate molecule could no longer fit, and the chemical change would be inhibited from taking place as efficiently or not at all. Every enzyme has an optimum pH range outside of which the enzyme is inhibited. Some enzymes like many of the hydrolytic enzymes in the stomach such as Pepsin and Chymotrypsin effective operate at a very low acidic pH. Other enzymes like alpha amylase found in the saliva of the mouth operate most effectively at near neutrality. I think that trypsin will work best at an alkali pH like 8 or 9. ...read more.

Middle

Outside that temperature range the enzyme is rendered inactive and is said to be totally inhibited. This occurs because as the temperature changes this supplies enough energy to break some of the intramolecular attractions between polar groups as well as the Hydrophobic forces between non-polar groups within the protein structure. When these forces are disturbed and changed, this causes a change in the secondary and tertiary levels of protein structure, and the active site is altered in its conformation beyond its ability to accommodate the substrate molecules it was intended to catalyse. Most enzymes (and there are hundreds within the human organism) within the human cells will shut down at a body temperature below a certain value, which varies according to each individual. In this case I think that it will work best a 400 C as this is about body temperature and it is at about this temperature that it works in the Duodenum. Preliminary experiment: We are investigating the best pH at which Trypsin works. ...read more.

Conclusion

A piece of the film was placed in each test tube. At this time a stop clock is started. Each test tube is monitored until the film goes clear. Results table: Analysis: The optimum pH of the enzyme was pH 9, which is what I predicted the preliminary experiment showed this. However the optimum temperature wasn't 400 C but it was 710C, which is not what I expected this again goes back to the lock and key hypothesis. The higher temperature gave the active site become more elastic to change shape to fit the substrate. My conclusions are that trypsin works best at an alkali pH and a temp of 710 C. Evaluating: In my experiment I think that we should of used a bigger range of temperatures because then we may have been able to see when the enzyme denatured. I also think for the preliminary experiment we could have tested more buffer solutions to see whether the enzyme would have worked better in more alkali solution. I think that my results support my conclusion and there weren't very noticeable anomalous. ?? ?? ?? ?? Lucy Jones Lucy Jones Pg1 ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. The aim of the experiment is to investigate the effect of temperature on the ...

    Enzymes are usually very large in comparison with substrate, so only small portion of amino acid residues are near or in direct contact with substrate in enzyme-substrate complex. Most of the enzyme involved in control and maintaining correct structural configuration of enzyme i.e.

  2. To investigate the affect of temperature on the enzyme trypsin.

    Thus the highest temperature will show the highest rate of reactivity, which is only true until the trypsin reaches the stated temperature. This is similar to the use of enzymes in industry, where a great mass of enzymes is added to the substrate at a high temperature so that there's a very fast (and short lived)

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work