Are all Saccharides Reducing Sugars?
I am going to do an experiment to find out if all saccharides are reducing sugars
Equipment
Test tube’s Galactose Sodium Bicarbonate
Maltose Benedict’s Solution
Sucrose Water Bath
Lactose Test Tube Rack
Glucose Hydrochloric acid
Method
I will first set up the experiment by placing 5 test tube’s in the test tube rack, in each separate test tube I will put a different saccharide and this will be measured to 2cm3 for each. I will then one by one add a dropper full of benedict’s solution to each test tube and then place the test tube into the water bath for 2 minutes; I will repeat this process for each sugar. After the two minutes are up I will take the test tube out of the water to check if it has changed colour and will record the results whether it has or not. If any of the sugars do not change colour then I will place the sugars that don’t change in fresh test tubes, I will then add 4/5 drops of hydrochloric acid and then place the test tubes into the water bath for 3 minutes. We then take the test tube out of the water bath and slowly add sodium bicarbonate a small amount at a time until it stops fizzing, After it has finished fizzing we then add a dropper full of benedict’s solution and place the test tube back in the water bath for 2 minutes, then we take the test tube out of the water bath and check to see if there has been a colour change, we then record the results.
