Chemistry Lab Chromatography

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Varij Gosine

Title- Separation of dyes in screened methyl orange by paper chromatography.

Introduction- chromatography is a technique used to separate the components of a mixture by making use of differences in solubility of the components. It can be used to separate such complex mixtures as the amino acids in blood or the various pigments in the flowers of a plant. In this experiment, the components of screened methyl orange which is a mixture of methyl orange (C14H14N3NaO3S) and xylene cyanol (C25H27N2NaO6S2) are separated through the use of water as a solvent.

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Aim-To determine which dye in screened methyl orange is more soluble in water.

Apparatus-screened methyl orange, chromatography paper (10cm X 2.5cm), 250cm3 beaker, fine dropper, retort stand.

Method-

  1. A horizontal line was drawn on the chromatography paper 1.5cm above the bottom line
  2. Some of the screened methyl orange was taken in the fine dropper and a spot of .5cm in diameter was made on the horizontal line.
  3. 20cm3 of distilled water was poured into the 250cm3 beaker
  4. The top of the chromatography paper was clamped to the retort stand and the lowered into the beaker ensuring that the spot ...

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