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How quickly does amylase break down starch when we change the temperature.

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Introduction

GCSE coursework science-planning strand Jack Darby 10F1 Miss Riaz Title of activity How quickly does amylase break down starch when we change the temperature. Instructions List I gathered the equipment shown below. I put 3 drops of iodine in each chamber of the spotting tile. I boiled the kettle and mixed together water from the kettle with water from the tap so the water reaches the correct temperature. The water was put into a beaker. 5ml of starch was put into a test tube. The test tube was placed into the beaker. When the water in the beaker was at the required temperature the stop clock was started. After one minute 1ml of amylase was put into the test tube with the starch using a syringe or pipette. As soon as the amylase and the starch had mixed a sample was taken from the test tube using a pipette and mixed with the droplets of iodine in one of the chambers of the spotting tile. ...read more.

Middle

I think that as the solution gets higher than that temperature the reaction will either be very quick or very slow depending on whether the reactants become denatured or not. I predict that the line on the graph will go up and then dip if and when the reactant denatures. Range of results I intend to take I intend to take results for 20,25,30,35,40,45,50,55,60 degrees Celsius. I will repeat my experiment. I think that there will be a wide range of results because as the temperature of the amylase changes the rate of reaction between the amylase and starch will change. I think there will be anomalous results because I think that at some point the reactants will denature and the reaction will be very slow. Scientific knowledge to explain your plan and prediction The amylase works best at around 37 degrees Celsius because that is body temperature. ...read more.

Conclusion

If the amylase has completely broken down the starch the iodine does not change colour when the amylase and starch solution is added to it. Diagrams to illustrate detailed science Explain how you will make sure your results are precise and reliable I will make sure my results are precise and reliable by recording my results accurately. I will make sure that the spotting tile and the test tubes are cleaned thoroughly after each experiment. I shall measure the temperature accurately and use the same equipment throughout the course of the experiments. I will make sure I am not biased when judging when the starch has been broken down. I shall repeat the experiment to ensure the results give a more precise indication of the reaction rate between amylase and starch. I will use a syringe instead of a measuring cylinder because this means that the measurements are much more reliable and accurate. I will use a thermometer which can measure temperature precisely. ?? ?? ?? ?? ...read more.

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