Proteins are a large number of organic compounds that make up living organisms and are essential to their functioning. Proteins are composed of units of about 20 different , which, in turn, are composed of carbon, hydrogen, oxygen, nitrogen, and sometimes sulfur.
Lipids are a family of naturally occurring compounds grouped together by their relative insolubility in water and solubility in nonpolar solvents. These are naturally occurring molecules that can be isolated from cells and tissues by extraction using nonpolar organic solvents, such as absolute ethanol. Lipids are hydrophobic. This group of molecules includes fats and oils, waxes, phospholipids, and some other related compounds. Fats and oils are made from two kinds of molecules: glycerol (a type of alcohol) and three fatty acids joined by dehydration synthesis.
Aim
To test whether five unknown substances contain starch, sugars, proteins or lipids, and then identify what the substances could be.
Apparatus
-oil
-alcohol (absolute ethanol)
-copper sulphate
-potassium hydroxide
-iodine solution
-benedict’s reagent
-bunsen burner
-tongs
-palette
-tongs
-matches
-pipettes
-test tubes
-unknown powder 1: fine yellowish powder
-unknown powder 2: fine white powder
-unknown powder 3: fine white power (like flour)
-unknown powder 4: white powder with rather large granules (like sugar or salt)
-unknown powder 5: thin white flakes (rather large particles)
Method
Starch: To approximately 4cm2 of substance add two drops of iodine solution. A blue dark colour indicates the presence of starch.
Sugars: To approximately 2 cm2 of unknown powder in a test tube, add an equal quantity of Benedict’s reagent. Shake and bring to boil by heating the test tube over a bunsen burner. A precipitate indicates reducing sugar.
Proteins: Add a little potassium hydroxide to the unknown powders till the solution clears, add a drop of copper sulphate down the side of the test tube. Do not heat. A blue ring at the surface of the solution indicates protein, on shaking, the blue ring dissapears and the solution turns purple.
Lipids: Shake some olive oil in a test tube with the unknown powder for one minute; add an equal amount of water. A cloudy white precipitate indicates lipids.
Each test should be carried out for each unknown powder and the results should be recorded.
Results
These were the results collected from each of the tests carried out.
Bibliography
-Encarta encyclopedia (CD-ROM)
-Biology for life (text book)