Investigate the effect of changing the hydrogen peroxide concentration on the rate of reaction of the break down of hydrogen peroxide by catalase.

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Planning Sheet for Science

Investigation

Problem/Aims

        My aims for this experiment are to investigate the effect of changing the hydrogen peroxide concentration on the rate of reaction of the break down of hydrogen peroxide by catalase.

Background Information

Predictions

        I predict that the higher the concentration of hydrogen peroxide is the more it will react with the catalase and therefor producing more oxygen. This is due to the fact that as the concentration of hydrogen peroxide is increased, the number of collisions between the enzyme (yeast) and substrate (hydrogen peroxide) increases. I believe that if I double the concentration of hydrogen peroxide, the rate of reaction will also double.

Key Factors

The main factors that I fell will influence the experiment are:

  1. Temperature of the catalase and hydrogen peroxide. As the more heat there is the more kinetic energy the enzyme and substrate will have causing them to move faster which will then cause more collisions and faster reactions rates.
  2. The concentration of hydrogen peroxide. The higher the concentration of hydrogen peroxide the more substrates there will be, which will enable more collisions between the hydrogen peroxide and catalase, therefore the reaction rate will increase.  
  3. The volume of catalase
  4. The amount of time the experiment will go on for
  5.  The apparatus I will use during the experiment. I must make sure I use the same apparatus each time.

The One factor that I will deliberately change.

  1. The concentration of the hydrogen peroxide. I will lower its concentration each time by adding it with measured amount of distilled water. I will change the concentration 11 times.

The factors that I will keep constant (Fair Test) 

  1. The am going to measure the total oxygen volume after two minutes for each change of concentration I make.
  2. I will keep the volume of the catalase (yeast) the same for each measurement I do. Which is going to be 5ml2
  3. The same concentration of catalase for each measurement I do. The concentration of the catalase is 2 mouler  
  4. The hydrogen peroxide I use will all have a concentration of 1 mouler, this is before I mix it with the distilled water.
  5. I am using distilled water throughout the whole experiment.
  6. I will keep the temperature of the distilled water, hydrogen peroxide and catalase the same.
  7. I will use the same apparatus throughout the whole experiment  
  8. I will not stir any of the solutions.
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Measuring/ Observing

        In this investigation I am looking to see if the rate of reaction changes. Measuring the total volume of oxygen produced in each experiment will give me a good indication to how much the reaction rate is taking place. So the main measurement I have to make is the volume of oxygen. I will do this by

Apparatus

        The apparatus I am going to write down I will use for each change of concentration I do. I equipment I will need for me to receive the most accurate data will be:

         Distilled water

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