Investigate the effect of light intensity and the colour of light on the rate of photosynthesis of Elodea.

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Hawa Mohamed-11x2

Science course work-photosynthesis

Aim: to investigate the effect of light intensity and the colour of light on the rate of photosynthesis of Elodea, (Canadian pondweed), to do this, I placed a piece of Canadian pondweed in varying light intensities, and observed the amount of oxygen being given off. I used Canadian pondweed because of its unusual quality of giving off bubbles of gas from a cut end, when placed in water.

Apparatus: to do this experiment I will be using the following equipment…

  1. 60 –watt bench lamp
  2. Canadian pondweed, (well illuminated prior to the practical)
  3. Colour filters- red, yellow, green, blue x 1mm thickness
  4. 500ml beaker
  5. Glass funnel
  6. Blue-tack (to hold the glass funnel from the base of the beaker)
  7. Stop watch
  8. Test tube
  9. Top pan balance
  10. Spatula
  11. Metre ruler
  12. Thermometer
  13. Clamp stand to hold colour filters in place
  14. Sodium hydrogen carbonate
  15. Pair of scissors
  16. 15cm ruler
  17. Glass rod
  18. Bucket of water of immersion
  19. Detergent (washing up liquid)

Prediction: I predict that as the light intensity increases so does the rate of photosynthesis. The reason is that when the light is increased, the energy absorbed by the chlorophyll increases, which makes the carbon dioxide increase as well. I also predict that as the distance of the light is away from the plant, the weaker the plant is. This is because as the distance doubles the number of bubbles decrease. For example, at 10cm, there will 25 bubbles but at 30cm, there will be 11 bubbles. I hypothesised that if the light intensity increases, the rate of photosynthesis will increase at a proportional rate until a certain level is reached, and the rate of increase will then go down. Eventually, a level will be reached where an increase in light intensity will have no further effect on the rate of photosynthesis, as there will be another limiting factor, in this case probably temperature. I also predict that blue colour will produced the highest rate of bubbles, and then red, and then followed by any of the other colours

  Method: plan of method is cut a stem of Canadian pondweed of about 3cm in length. Fill a test-tube with pond water, and place it in a clamp, and then in a large beaker of cold water. Connect the end of the pondweed to the apparatus. Insert a thermometer into the beaker, and record the temperature at the beginning and end of each experiment, merely as a safety measure against an important rise in temperature, which is not expected. Set up a lamp at a set distance from the plant, making sure that this distance is from the filament of the lamp to the actual pondweed, rather than the edge of the beaker, and assumed the same at any point at any particular distance. When bubbles are, being produced at a steady rate, clear any previous bubbles from the tubing by moving the syringe. Start the stopwatch, and wait for 1 minute. Move the bubbles, which have been collected at the bend in the tubing to the part of the tube with a scale. Find the length of the bubble collected. Repeat for all other readings,

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    Sciencetific knowledge: Only green plants can make their own food by the process called photosynthesis. Hence, green plants are known as food producers. All other organisms are called food consumers as they depend directly or indirectly on green plants for food.

Importance of Photosynthesis

Photosynthesis is a very important process for the following reasons:

* During photosynthesis, green plants trap the energy from the Sun. This energy is passed on to all other organisms that depend directly or indirectly on green plants for food.

* Photosynthesis replenishes the oxygen that has been used up by all organisms during respiration.

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