From my research I predict that more gas will be produced but only when the temperature is close to body temperature. Enzymes work best at body temperature and become denatured at higher or lower temperatures. This causes me to think that as the temperature increases to over 50 degrees. But, once the temperature of the hydrogen peroxide begins to rise past 50 degrees, I thin that the amount of gas produced will decrease. I think this will happen because at high temperature the enzymes will lack in their work and wont react properly because of the collision theory.
Key Factors:
The temperature the hydrogen peroxide will be heated.
The amount of either liver or the hydrogen peroxide.
The amount of time that the liver and hydrogen peroxide
is left to react.
Method:
We gathered our equipment and set it up accordingly. We then measured five grams of liver and twenty millilitres of hydrogen peroxide several times and set them up aside in separate beakers. We filled a measuring cylinder (100ml)full with water and stood in place upside-down using a clamp and stand. We connected the tube in side the upside-down beaker that leads to a bung. Then we heated the hydrogen peroxide to the temperature we were testing. We had to do the test five times not including repeats. The we put the liver and the heated hydrogen peroxide in the bung together and waited for one minute for a full reaction. The we recorded the amount of water missing from the beaker i.e. the gas made from the reaction and escaped. The we did the other temperatures and the repeats.
Results:
Method 2:
We prepared the equipment and filled a beaker with hydrogen peroxide but at very accurate temperature measurements. Then we weighed the beaker and subtracted that from the total weight of the hydrogen peroxide and liver (separately). Then we added the two together and left to stand for two minutes. After the time we weighed it again to see if any mass had been lost during the reaction. We then did this again but with different temperatures and repeats.
Results:
Conclusion:
My results show me that the higher the concentration of a substrate, the quicker the reaction rates of the substrate and the enzyme working on it. The 100% concentration produced the most o2 in the shortest time, which gives it a higher reaction rate than the others. My prediction for the first experiment was the same as the second one and for this experiment I was correct. Because in the results the first two leads up to the highest reading which is the 3rd then it lowers, this shows that the enzymes are denaturing by the collision theory.
My results were pleasing for the second method. The results in this test were very accurate and precise. The original results and the repeats were near enough the same. To make the test fair I didn’t round up the temperatures but I got them as close to the targeted temperature. Because in these two tests the results were pleasing for they did follow the collision theory rule, I will use the same measurements for my write up.
