Testing For Non-Reducing Sugars

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Testing For Non-Reducing Sugars

Aim: To test for non-reducing sugars 8 solutions of Glucose and compare their colour change compared with 3 different types of everyday liquids that contain sugar content.

Equipment: The Equipment that will be needed for this experiment is:

  • Spatula
  • A sample to test: the sucrose solution
  • Test tube holder
  • 1 mol per dm hydrochloric acid, sodium hydrogen carbonate
  • Benedict’s solution
  • Pipette
  • Water Bath tank
  • Eye protection
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Method: For this experiment I am going to:

  • Measure 5cm of Sucrose Solution into a boiling tube and add 1cm of dilute Hydrochloric Acid (HCl)
  • Stand the Tubes in a Boiling Water Bath by using a rack and leave for 5 Minutes. Remove the Rack with all the Test Tubes and leave to cool for a short period of time.
  • Carefully add Sodium Hydrogen carbonate to the solution in the tubes, this should cause a reaction to occur where the solution fizzes. Add small amounts of Sodium Hydrogen carbonate until the fizzing eventually stops.
  • Now add 3cm ...

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