Diffusion
The molecules are all separate
The molecules have diffused
Variables in my investigation: The following are variables in my investigation. All these experiments show different ways of finding out about the rate of diffusion.
The temperature of the agar For this experiment you would change the temperature of the acid so that one agar block was diffused at 0°C, another at 10°C and onwards, changing by 10°C each time.
The surface area of the agar For this experiment you would change the surface area of the agar so that the size varied. You would then investigate and see if the diffusion rate changed.
The concentration of the HCL For this experiment you would change the concentration of the acid by adding more or less water: for example:
water acid
0 10
- 8
4 6
6 4
- 2
The variable I will be changing is the concentration of the acid.
Prediction: I predict that the higher the concentration of acid in my experiment, the quicker diffusion will take place. My reasoning for this is that diffusion takes place quicker with stronger substances, hence, the stronger, or the higher the concentration of acid, the quicker diffusion will occur.
Appartus: The apparatus I will be using is listed below:
Test tubes – I will put the agar and HCL in these
Test tube rack – These are to hold the test tubes
HCL – This stands for hydrochloric acid, the acid I will be using
Water – This is to dilute the acid
Measuring cylinder – I will use this to measure the volume of acid
Stopwatch – This will be used to time the diffusion rate
Forceps – I will use the forceps to pick up the agar
Agar – Agar is the substance we will be using to see how quickly diffusion takes place. Agar is a jelly-like substance, it is orange but as acid slowly diffuses through it, it turns orange. This is a very useful property of agar as it means it is easy to spot when diffusion has finished. If the agar is touched by hands it will become heated and contaminated, it must also not be exposed to acid before the actual test.
My method: Below is the method I will use to complete my investigation.
- Firstly I will pour the correct amount of acid and water (using the measuring cylinder to get the amount accurate) into the test tube. water acid
0 10
2 8
4 6
6 4
- 2
- Next I will put the agar into the test tube filled with acid, using the forceps.
- When the agar is in acid I will start a stopwatch and record the time accurately.
- I will continue to do this with different concentrations of acid.
- I will record each 5 times to get a fair result and average.
Safety points: Below are some points I must take notice of when carrying out my investigation.
- Wear safety goggles
- If the agar is touched by hands it will become heated and contaminated, it must also not be exposed to acid before the actual test.
- Use forceps to move the agar
- Have a blackboard behind the test tube to see when the colour change, and diffusion, takes place.
-
Measure the acid and water carefully.
Setting up a fair test: To make sure my test is fair I will record each result 5 times, to be able to make an average for my graph and to be able to analyse my results. My agar blocks will also be the same size (1cm³) and the temperature of the experiment will be the same, room temperature (roughly 27°C).
Results: Below are my results table and the results I gathered from my investigation.
For my results in a graph look at the next page.
acid water TIME minutes average
10 0 4:30 4:41 4:42 4:37 4:35 277secs
8 2 5:37 5:44 5:35 5:32 5:41 335secs
6 4 *5:16 *5:18 *5:09 *5:12 *5:15 314secs
4 6 6:23 6:33 6:27 6:25 6:35 388secs
2 8 8:08 8:05 8:10 8:02 8:12 487secs
*This shows that these results may not be reliable, as they are a shorter time than the result row before, but according to the rest of the table, should be longer than the results before. I am not sure these results are correct.
Analysis of Data: My results seem to be accurate and turned out as I expected although the results for the ‘6 acid 4 water’ were anomalous, these results were anomalous because they did not correspond with the rest of the results in any way. I think the reason for this anomaly could have been that the size of the agar was not quite accurate or that the concentration of the acid was not correct.
Conclusion: I can prove from my results and research that diffusion takes place quicker if the concentration of acid is higher. I therefore conclude that the lower the concentration of HCL, the time taken for diffusion to occur will be longer. The molecules moved faster the higher the concentration of acid, making more collisions. Because of this the rate of diffusion is quicker, this means that the acid diffused through the agar.
Evaluation: I feel that my experiment went well though if I had had a chance I would have spent more time on it making sure my results more accurate and my practical better. I think my data was reliable because there was one anomaly, it was sufficient enough for me to draw a conclusion. To see if diffusion happens with different variables I could test:
The temperature of the agar For this experiment you would change the temperature of the acid so that the agar blocks were diffused at different temperatures, increasing by 10°C each time.
The surface area of the agar For this experiment you would change the surface area of the agar so that the size varied.
JAMES LAGDEN 9 SEB