To investigate the effect of water concentration on the mass of potato tissue.

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Biology Osmosis Coursework

Aim:

To investigate the effect of water concentration on the mass of potato tissue.

Scientific Theory:

Osmosis is defined as the movement of water molecules across a partially permeable membrane from a region of high water concentration to a region of low water concentration. In a high concentration of water the amount of solute (e.g. sugar) is low. This could be called a weak or dilute solution. In a low concentration of water the amount of solute (e.g. sucrose) is high. This could be called a strong or concentrated solution. When two such solutions are divided by a partially permeable membrane the water will move from the area of high concentration to the area of low concentration, until both sides are equal (have reached equilibrium).

Knowing that osmosis will occur across a partially permeable membrane whenever there is a difference between the water concentrations on the two sides of the membrane, and knowing that when this happens to cells they will either become turgid if water flows into them, or flaccid if water flows out of them, and thus change their volume, we want to test the hypothesis that:
If the concentration of a solution into which a cylinder of potato is placed is greater than a certain level the cylinder will contract, and if the concentration is less than that level it will expand. This can be seen in living cells. The cell membrane in cells is partially permeable and the vacuole contains a sugar/salt solution. So when a cell is placed in distilled water (high water concentration) water will move across the partially permeable membrane into the cell (lower water concentration) by osmosis, making the cell swell. This cell is now referred to as turgid. If done with potato cells the cells would increase in volume and mass because of the extra water. If these potato cells were placed in a solution with a low water concentration, then the opposite would happen. Water would move out of the cell into the solution. In extreme cases the cell membrane breaks away from the cell wall and the cell is referred to as flaccid. The potato cells will have decreased in volume and mass.

The greater the concentration of water in the solution the greater the amount of water that enters the cell by osmosis. The smaller the concentration of water in the external solution the greater the amount of water that leaves the cell.

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Evaluation:

I was pleased with the results that I obtained from my experiments; they proved to be accurate as my repeat readings were very close to each other, there were also, as far as I can tell, no anomalous results. I did not encounter any anomalous results (An anomalous result being a result that does not agree with the general trend of the other readings) and therefore did not have to remeasure points. There is however, always room for improvement. Firstly, I did not take into account the effect of fluctuations in the room temperature on my experiment, though I ...

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