Aim: To investigate whether the temperature effects the production of yeast and hydrogen peroxide by measuring the oxygen gas given off for each temperature.

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Enzyme experiment

Aim: To investigate whether the temperature effects the production of yeast and hydrogen peroxide by measuring the oxygen gas given off for each temperature.

Catalysts are molecules which speed the rate reactions. Enzymes are biological catalyst which can be found in plants or animals. Catalase is an enzyme produced in the body to break down Hydrogen peroxide to produce oxygen and water (2H202 - 2H20+02 ).  

Hypothesis: I predict that as temperature rises, then there will be more oxygen gas bubbles given off as all chemical reactions speed up as temperature is raised. This is partly due to more molecules have enough kinetic energy to undergo the reaction and also enzyme reactions also tend to proceed faster with increasing temperature.

However, when the temperature of an enzyme-catalyzed reaction is raised further, an optimum temperature will be reached so beyond this point, the kinetic energy of the enzyme and water molecules is so great that the structure of the enzyme molecules will start to disrupt. Enzymes will start to denature and this destroy the enzyme’s ability as an active site.

Therefore, I predict that the optimum temperature of the reaction is around 45˚C and anything beyond that, the oxygen bubbles will decrease.

In order to keep the investigation fair, I will need at least 4 control variables and other variables like independent variable which is the data that I need to collect and the other dependent variables. These variables are:

Control variables:

  • 10ml  of hydrogen peroxide
  • 1ml of yeast solution
  • Same length of time recorded for each experiment for 1 minute each to measure the bubbles produced
  • Temperature needs to be maintained at given temperatures
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Methods of keeping the control variables controlled:

I will use a water bath to help maintain the desired temperature of hydrogen peroxide. If the thermometer indicates the temperature is decreasing, then I will add more hot water to the water bath in order to sustain the temperature.

In order to keep both the amount of hydrogen peroxide and yeast solution the same, I will be using a 1ml pipette and 10ml of measuring cylinder. This is to make sure that I get the correct measurement of yeast and hydrogen peroxide so that the investigation will be fair. ...

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