Biology IA - Paper Chromotography lab report

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Biology HL

Candidate name: Ieva Drūlytė

Date: ___________________

Separation of nettle’s photosynthetic pigments (Carotene, Phaeophytin, Xanthophyll, Chlorophyll a, Chlorophyll b) by paper chromatography

CE

Aim: to separate and identify photosynthetic pigments (Carotene, Phaeophytin, Xanthophyll, Chlorophyll a, Chlorophyll b) of nettle by paper chromatography putting the chromatographic paper into organic solvent – kerosene.

Hypothesis: plants photosynthetic pigment chlorophyll consists of five different pigments that differ in solubility in the solvent thus each move up the chromatography paper at different rates. Also, each pigment has a different shade of color.

Data collection and processing

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Table 1 Raw date - distances moved by the photosynthetic pigments and by solvent during 3 trials.

The pigments can be identified by their colors and Rf values. In the table above the names of pigment are written as the colors in all three attempts matched with original pigments’ colors.

To get Rf value the calculation is: Rf = a/b where -

a = distance moved by pigment from its original position;

b = distance moved by solvent from same position.

The calculations made to convert raw date into Rf value: (([1] + [2] + [3]) / 3) / [4]

Table ...

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