Chromatography lab. Title: separation of pigments of photosynthesis using paper chromatography.

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Title: separation of pigments of photosynthesis using paper chromatography.

Goal (main aim):

Calculating the Rf of every single pigment, in order to distinguish it and identify its solubility.

Hypothesis:

In photosynthesis; two types of pigments are involved; chlorophyll (A or B) and carotenoids, by using paper chromatography we can distinguish each pigment, by measuring the color's Rf; referring to the next formula:

 

Variables:

Independent variable:

  • Pigment solubility.

Dependent variable:

  • The distance that the pigments move, on the chromatography paper.

Controlled variables:

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Materials and procedure:

Materials:

  1. Chromatography Jar.
  2. Plant.
  3. Chromatography paper.
  4. Solvent (organic).
  5. Tweezers.
  6. Pipette.
  7. Mortar and Pestle.
  8. Scissors.
  9. Ruler.

    10- Calculator.

Procedure:

  • Preparation of the mixture:
  1. Place a piece of the obtained plant leave into the pestle.
  2. Add 5ml (approx.), of 90% isopropyl alcohol.
  • Preparation of the chromatogram:
  • Attain a chromatography paper.
  • Cut the paper, in order to have a triangular end.
  • Draw a line above the triangular end, with 1 cm, draw a point in the center of that line, the previous line is considered the start line.
  • By ...

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