Biology Lab: Extraction of Crude DNA from Plant and Animal TissuesBy: XClass: IB1Date: Friday, May 15, 2009 Background and Purpose:- In this experiment you will be extracting DNA from two sources: animal tissue (liver tissue) and plant tissues (kiwi). Two procedures will be followed and instructions must be followed precisely in order to obtain appropriate results.- The purpose of this experiment is to strengthen and acquire skills of measuring, observing, predicting, collecting and recording data. We will also learn how different solutions and substances lead to the extraction of DNA.Procedure 1: Extracting DNA from Fresh Sheep LiverMaterials: - Fresh Sheep Liver                      - Liquid Soap                 - Scalpel or Dissecting Scissors   - 95% ethanol                 - Blender                                       - 0.9% NaCl solution (saline)                 - Graduated Cylinders                 - Glass Stirring Rod    
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            - Droppers                 - Beakers                 - CheeseclothProcedure and Explanations:1- Cut a piece of liver about 2 cm long and place it with 30 ml of saline solution in a Blender. Blend till the mixture is uniform. The saline solution makes the positively charged phosphate groups in DNA neutral, thus instead of repelling each other they are drawn closer together. This leaves DNA in the solution and causing all other proteins and carbohydrates to precipitate.2- Fold two pieces of cheesecloth in half and use it to strain the liver mixture into a beaker.3- Use a dropper to add approximately 30 ml of ...

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