Osmosis Lab. Aim: To investigate the effects of different solute concentrations on osmosis, calculate water potential, and plant cell plasmolysis.

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Osmosis Lab

Aim: To investigate the effects of different solute concentrations on osmosis, calculate water potential, and plant cell plasmolysis.

Hypothesis: It is hypothesized that if in osmosis, water were moving out of the cell into higher concentrations then when the osmotic pressure increases the mass of the cells would shrink and decrease. I believe that the solutions with the higher amount of sucrose solutions will end up having the highest osmotic pressure along with the smallest mass meaning that the cells went through osmosis and shrunk the cells of the potato core.

Variables:

        Independent/Experimental: Size of Potato Core

        Dependent: Change of Mass

        Controlled: Measuring Mass, Measuring Temperature

Procedure:

  1. Cut 4 potato cylinders with the cork borer for each solution that you will use, and then cut them evenly so that they all are 3 cm in length. Remove any potato skin.
  2. Pour 100 mL of each sucrose solution that has already been prepared (water, 0.2M, 0.4M, 0.6M, 0.8M and 1.0M) in a cup and label it.
  3. Add 4 potato cylinders to each solution cover it and let it stand overnight.
  4. Tomorrow, remove the potato cylinders from each solution and blot them with paper towel.
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Data Collection and Processing:

        For the experiment, data was taken from six different samples. We cut six of the same size potato cores and measured the mass. After we measured the temperature of each cup filled with water and the solutions. Five of the cups had a different amount of sucrose solution and the sixth cup was just filled with water and its constant. Placing the potato wedges in the different solutions, the osmosis began to work and I left the cups sealed for a day. The next day, the temperature was measured again and it would stay ...

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