Research Question: Which method of DNA extraction (using a centrifuge or not) will yield the most DNA, and which fruit (banana, papaya, or strawberry) contains the most DNA?
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Introduction
DNA Extraction Lab Introduction Research Question: Which method of DNA extraction (using a centrifuge or not) will yield the most DNA, and which fruit (banana, papaya, or strawberry) contains the most DNA? The aim of this investigation is to extract DNA from three organic sources using two methods of extraction in order to determine how much DNA is contained in each source and which method is most effective in extracting DNA. For this investigation, the three sources of DNA are papaya, strawberry, and banana. The difference between the two methods of DNA extraction involves the use of a centrifuge. Hypothesis and Prediction The method involving the centrifuge will yield the most DNA, as it will allow the cell contents of each organic source to separate better than if they were just left to sit. I also predict that the papaya will yield the most DNA, as it appears to be the most genetically advanced. Variables Independent variable: type of fruit used and the use of centrifuge in the extraction process Dependent variable: amount of DNA ...read more.
Middle
11. Carefully wind the DNA in the solution around the stirring rod, making sure to extract all of it. 12. Record the mass of the stirring rod, with the DNA. The difference is the amount of DNA extracted. 13. Repeat the procedure five more times, being sure to use one sample of each fruit without the centrifuge and one sample of each with the centrifuge. Data: Extraction Without Centrifuge Fruit Type Mass of Stirring Rod (grams) Mass of Stirring Rod with DNA (grams) Amount of DNA extracted (first mass subtracted from second mass) (grams) Banana 52.1 57.5 4.3 Papaya 50.8 58.9 4.7 Strawberry 54.9 50.2 3.9 Average 4.433 grams Extraction With Centrifuge Fruit type Mass of Stirring Rod (grams) Mass of Rod with DNA (grams) Amount of DNA extracted (grams) Banana 55.1 58.3 6.4 Papaya 58.3 50.9 7.0 Strawberry 58.4 59.3 6.2 Average 6.533 grams Calculations To determine amount of DNA extracted, I subtracted the mass of the stirring rod from the mass of the stirring rod with the DNA. ...read more.
Conclusion
Statement of improvement: This lab could be improved by using a better filtering system. The coffee filters left too much liquid with the solid it strained. Perhaps a stronger filter would be better, as the remaining solid could be pressed and yield more liquid. The DNA invariably contained fluid when it was extracted, so drying the pure DNA before recording its mass would remove that extraneous mass. Utilizing a blender or another more efficient method of pulverizing the fruit would yield more DNA, as it would break all cells. Learning Statement: What I learned from this experiment was that the inclusion of one additional step (with the centrifuge) could have a profound effect on the amount of DNA yielded. I learned that the centrifuge separates the contents of a solution far more effectively than letting the solution sit. I also learned that polyploidy plays a role in the amount of DNA contained in each fruit, as the fruit with the most chromosomes yield the most DNA. This experiment shows the importance designing an experiment with the right procedures, as the omission of one step produced a much lower percent yield of DNA. ...read more.
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