The Effect of pH on the activity of enzyme Catalase

Authors Avatar by sharmisthac (student)

Effect of pH on the activity of the enzyme catalase                  Riddhi Draksharapu

The Effect of pH on the activity of enzyme Catalase

Enzymes are globular proteins responsible for most of the chemical activities of living organisms. The pH at which enzymes function are extremely important.

Hydrogen peroxide (H2O2) is toxic to most living organisms. H2O2 can be converted to oxygen and water, as follows:

H2O2                                      2 H2O+O2

Although this reaction occurs spontaneously, enzymes increase the rate considerably.

In this experiment, rate of enzyme activity   was measured  under  different pH values.


  1. Most   enzymes have an optimum pH near to 7. This is the optimum pH found inside most of the living cells.
  2. pH is the measurement of the concentration of the Hydrogen ions (H+)
  3. Hydrogen ion will affect the hydrogen and ionic bonds within the enzyme.
  4. If these bonds are changed the three dimensional shape is changed altering the shape of the active site.
  5. When the enzyme shape is altered, it becomes denatured.
Join now!


Table 1:  Variables Selected for this Experiment

Materials and Methods:

  1. Fresh potato
  2. Boiling tube -1
  3. Single bore rubber bung
  4. Cork borer
  5. White tile
  6. knife
  7. Small beaker
  8. 2x10 cm3 measuring cylinder
  9. Manometer tube (3mm in diameter)
  10. Stop watch
  11. Forceps
  12. Ruler
  13. 20 cc hydrogen peroxide
  14. Range of buffers (pH-3-8)


  1. Use a cork borer to cut cylinders of fresh potato tissue between  1 cm in length. Place on the tile to cut into at least 30   discs prepared for replicas for each sets each 1 mm wide.
  2. Place all ...

This is a preview of the whole essay