The variation of rate of enzymatic activity of Bos primigenius liver with respect to surface area

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The variation of rate of enzymatic activity of Bos primigenius

liver with respect to surface area

Introduction

In this lab the rate of enzymatic activity will be tested by pouring hydrogen peroxide over cow liver. A reaction will take place its products are water and oxygen. The enzymatic activity rate will be compared between pieces of liver with different sizes, and the change in pressure in a bottle/container will be measured, which will indicate the enzymatic activity rate.

Materials:

  • Device measuring pressure
  • Liver
  • Nalgene reaction bottle
  • Syringe without a needle
  • 3% solution of hydrogen peroxide
  • Scalpel
  • An electric scale
  • Tweezers
  • Computer with Logger Pro installed

Independent Variable:

  • Mass of the liver piece

Dependent Variable:

  • Change in pressure in the nalgene reaction bottle

Controlled Variables:

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Procedure:

  1. Set up the device for measuring the pressure
  1. Assemble it
  2. Plug it into the computer and open Logger Pro 3
  3. Set up an “experiment” in the program for 60 seconds, data taken every second
  1. Cut 12 pieces of liver
  1. 3 pieces of liver with the mass of 1g, 1.5g, 2g and 2.5g
  1. Put a piece of liver into the pyramid-like tube
  2. Load the syringe with 15 mL of hydrogen peroxide
  3. Press “start experiment” in LogoPro and pour the hydrogen peroxide into the nalgene reaction bottle and ...

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