The X-value represents the numbers from the samples collected from the recordings of the samples.
Table 2 (the recordings of the Y-values from both tests):
The Results from the samples:
Test 1:
Sample 1 : 0.06
Sample 2:
Sample 3:
Sample 4 :
Test 2:
Sample 1: or
Sample 2:
Sample 3:
Sample 4: 0.06
Here are the actual Rf-Values for each amino acids (theoretical value):
Alanine – 0.38
Lecine – 0.73
Lysine – 0.14
Conclusion and Evaluation:
Conclusion:
Based from our results, we see that Sample 1 is a mixture of leusine and Lysine since the Rf value of sample 1 is the same as sample 4. The theoretical results that are above are clearly conceded with the experimental results that we got in this experiment.
Based from the Rf values we could see that the alanine is non-polar molecule with a basic PH value, leucine is a polar molecule with a basic and the lysine is a non-polar molecule with a neutral PH value. Certain variables that affect the Rf values, could be the polarity of the different amino acids. The longer the distances is on the sample, it is most likely that the amino acids is polar, while is the distance of the Rf value is shorter, it is most likely that the amino acid is un-polar. That can be due the length of the Carbon chains, Hydrogen Bonds and van der walls forces in the amino acids. If there carbon chains are shorter then the amino acids are Non-polar, while there is longer chains of carbons the amino acids is polar. Also the amount of butanol in solvent putted into the amino acids can be a factor to if the molecules are polar or non-polar.
Evaluation:
The experiment went well, but the experiment could be improved. During the experiment, there were some human errors when checking the time and adding the concentrated polar solution off butanol. We didn’t have the proper equipment to measure the precise measurements of the amino acids. This experiment could be improved by:
- Repeating the experiment
- Getting proper equipment for the experiments
- Fewer human errors
- Getting a more Un-polar solution of Butanol.
Appendix:
Picture 1(a image showing the results of the tests):