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University Degree: Applied Biology

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  1. Isolation & Characterisation of Proteins

    Distance migrated (cm) (Distance migrated)-1 Size of fragments (kb) 1.5 0.667 25.05 2.2 0.455 15.87 2.7 0.370 12.19 3.1 0.323 10.16 3.8 0.263 7.56 5.5 0.182 4.06 11.2 0.089 0.04 Calculation: To calculate for the fragment size of DNA fragment digested by HindIII, using the equation from the graph above: y = 0.0231 x + 0.0883 Let y be the (distance migrated)-1 and x be the size of fragment, When y = 0.667 0.667 = 0.0231x + 0.0883 x = = 25.05 kb When y = 0.455 0.455 = 0.0231x + 0.0883 x = = 15.87 kb When y =

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