To investigate the effects of abiotic factors specifically pH on the abundance of marram grass.

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Sneha Patel        Page         

Aim: To investigate the effects of abiotic factors specifically pH on the abundance of marram grass.

Succession is the gradual change in an inhabitable terrain by which organisms are changed and replaced eventually forming a climax community habitable by many organisms.

Primary succession begins with a barren terrain is inhabited by a pioneer species which colonises and allows the once inhabitable land to be occupied by other organisms. The succession I will be looking at will begin with a sandy environment, a psammosere. The main pioneer specie here is marram grass (A.arenaria). Pioneer species often have many adaptations that allow them to colonise in an environment that many other organisms couldn’t. Marram grass is not different. As it colonises in a psammosere it has many xeromorphic adaptations. The plant is able to stabilise the sand and as it decomposes, it is able to provide nutrients to sand allowing it to become inhabitable by other plant species. The ability of a pioneer species such as marram grass to germinate grow and reproduce is called an Ecesis. Marram grass is a R-selected species. This means that in it’s short lifetime it will reproduce a lot.

During my experiment I will be investigating the effects of abiotic factors, specifically pH on the percentage abundance of marram grass.

Null hypothesis: there is no correlation between pH and the abundance of marram grass

Alternative hypothesis: there is a correlation between the pH and the abundance of marram grass.

I predict that there will be a positive correlation between the pH level and the abundance of marram grass. I believe this, as marram grass is not adapted to acidic conditions. As we get further away from the beach I expect the number of marram grass to decrease, as the sand gets more acidic. I believe that the sand will get more acidic as we get further from the sea as the amount of humic acid present in the sand will also increase. The humus is formed form the decomposition of marram grass. This will decrease the pH levels, as more H+ ions will be in the sand.

Method:

  • To find the profile on the sand dune we will be using the line transect and frame quadrant method.
  • The reference pole(C) should be placed in the distance and will be used to align the other to poles.
  • The two other poles (A and B) will need to be separated by 10 meters using the string attached to a spirit level.
  • Now place the 50cm² quadrant at the base of A.
  • After placing the quadrant at a right angle to the pole count the number of organisms in each square. Record this on a table.
  • Using the thermometer measure the temperature by placing it into the ground. Similarly using the moisture probe, place it into the ground and record the moisture level.
  • To measure the infiltration, stick the tube into the ground. Pour 200 ml of water into the tube and time how long it takes for all the water to be absorbed.
  • By using the anemometer measure the wind speed by holding it in the air in the direction of where the sea is. Wait for a few seconds and then record the reading.
  • Finally to measure the pH collect a sample of soil in a test tube, add a few drops of universal indicator and shake it. Compare the colour to the chart and then not down it’s pH.

Apparatus

  • 3 ranging poles
  • Spirit level and 10m string
  • 50cm² quadrant
  • Tape measure
  • Digital thermometer
  • Anemometer
  • Moisture level probe
  • Stopwatch
  • Small tube
  • 1 litre measuring cylinder
  • Spatula
  • Test tube
  • Universal indicator
  • Identification key
  • pH level identification key

Safety

There are certain safety issues that need to be considered before doing this experiment:

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  • The digital thermometer and anemometer have a sharp point, when carrying these apparatus they should be held vertically
  • Similarly the 3 ranging poles are also dangerous as they are big and have a sharp point. Again they should be held vertically when carried
  • Also when walking through the dune. There may be wild animals and the terrain may be extreme so suitable footwear and extra caution should be taken.

Analysis

Table 1

A results table to show the effect of abiotic factors on the abundance of marram grass

Table 1

Table 1 shows the ...

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