An investigation to determine the effect of temperature on the permeability of beetroot cells.
Katie Ruck
An investigation to determine the effect of temperature on the permeability of beetroot cells
Evaluation
Although an apparent trend is illustrated by the experimental data plotted, I am reluctant to formulate a valid conclusion on the effect of temperature on the permeability of beetroot cells due to the variability of the results obtained.
Although five repeats were performed, the data collected is not reliable because of variation within the sets of results. This could have been due to various limitations of the experiment. At 30oC for example, the reading for absorbance of light in arbitrary units, was 0.12%. When compared to the results collected from other repeats at this temperature, this appears to be an unusually high value. Further examples of possible anomalous data were 0.03% at a heat treatment of 40oC together with 0.06% at 50oC. If these anomalous results were not included in the mean absorbance plotted, this could have had a significant effect on the overall conclusion. For example, had the reading at 40oC not been included in the mean, the reading plotted at this temperature of heat treatment may not have been lower than the mean result plotted at 30oC, as is shown on the graph by a slight dip.
An investigation to determine the effect of temperature on the permeability of beetroot cells
Evaluation
Although an apparent trend is illustrated by the experimental data plotted, I am reluctant to formulate a valid conclusion on the effect of temperature on the permeability of beetroot cells due to the variability of the results obtained.
Although five repeats were performed, the data collected is not reliable because of variation within the sets of results. This could have been due to various limitations of the experiment. At 30oC for example, the reading for absorbance of light in arbitrary units, was 0.12%. When compared to the results collected from other repeats at this temperature, this appears to be an unusually high value. Further examples of possible anomalous data were 0.03% at a heat treatment of 40oC together with 0.06% at 50oC. If these anomalous results were not included in the mean absorbance plotted, this could have had a significant effect on the overall conclusion. For example, had the reading at 40oC not been included in the mean, the reading plotted at this temperature of heat treatment may not have been lower than the mean result plotted at 30oC, as is shown on the graph by a slight dip.