Formulations used
Commercial formulations subject to dissolution testing includes
Panadol actifast (£2.74 : 20 p per tab)
500mg paracetamol,
Sodium bicarbonate, soluble starch ,povidone (gelling agent), maize starch(disintergrant and anti adherent), potassium sorbate (E20), microcrystalline cellulose (binder), magnesium stearate(lubricant and antiadherent), carnauba wax , titarium dioxide (E171), polydextrose, hypromellose, glycerol triacetate and polyethylene glycol(solution binder).
Anadin (£1.99 : 12 p per tab)
500mg paracetamol,
Croscarmellose (super disintergrant) sodium, povidine(viscosity enhancer), pregelatinised maize starch(disintergrant), stearic acid (lubricant), hydroxypropyl methyl cellulose (E464) (solution binder), polyethylene glycol(solution binder).
Paracetamol galpharm (£0.16p: 1p per tab),
500g paracetamol,
Pregelatinised maize starch BP(disintergrant),, sodium metabisulphide BP and magnesium stearate BP(lubricant).
Capsules manufactured from experiment 3 contained:
DISSOLUTION STUDIES ON THE MANUFACTURED CAPSULES
FORMULATIONS TESTED
- Starch 1500( vessel 1 and 2)
- Microcrystalline cellulose ( vessels 3 and 4)
- Lactose (vessel 5 and 6)
* indicate solution is diluted by 20 fold
TABLE 1: ABSORBANCE OF VARIOUS FORMULATIONS CONTAINING DIFFERENT DISINTEGRANTS AT VARYING CONCENTRATIONS MEASURED AGAINST TIME.
Using the values obtained above, the amount of paracetamol dissolved in a one litre solution was obtained by extrapolating from the calibration curve. The calibration curve gives the concentration in mg/l. This is converted to in 900 ml by.
Example 0.745 read of the curve gives a concentration of 15.5 mg/l. Hence In 900ml (0.9L),
Xmg= 0.9 x 15.5
1L
Xmg = 13.95 mg
For solutions with asterisks * the concentration is multiplied by 20 after being found how much is in 0.9L.
The values are shown below (table 2)
The expected normal weight is 25mg per capsule: To calculate % of drug released in solution, is the amount weighed in solution divided by expected weight multiplied by 100%. Example is percentage of in panadol five mintes = 13.95 x 100% = 55.8% 25
COMMERCIAL FORMULATIONS TESTED
- Panadol actifast ( vessel 1 and 2)
- Anadin ( vessels 3 and 4)
- Paracetamol galpharm(vessel 5 and 6)
For tablets, all solution has been diluted by 20 fold.
To calculate the concentration, in 0.9L the concentration is read of the calibration curve; example 0.841 read off the graph is 15.45 mg/L. This is then multiplied by 20 (dilution factor). 15.45 x 20= 345mg/L : in 0.9L; 0.9 x 345= 310.5mg.
The expected normal weight is 500mg: To calculate % of drug released in solution, is the amount weighed in solution divided by expected weight multiplied by 100%. Example is percentage of in panadol five mintes = 310.5 x 100% = 62.1%
500
RESULTS AND DISCUSSION
Figure 1 is a graph that shows the % of drug content released with time for the paracetamol capsules which differed in terms of the diluent used. When comparing the formulations it is evident that the one lactose had the fastest dissolution rate as it released its total quantity of drug encapsulated into solution within 45 minutes, followed by the starch 1500 formulation and then by the microcrystalline cellulose formulation with released its totally quantity of drug into solution after 60 minutes.However the percentage of drug released being over 100% suggests that perhaps the capsule didn’t contain the correct quantity of drug to begin with this could be due to poor mixing of powder , possibly due to poor filling of the capsule due to nature of powder mix or inefficiency of machine . Moreover, these results suggest that the presence of lactose in a paracetamol capsule formulation enhances its dissolution rate, more so than either starch 1500 and microcrystalline cellulose. this could be because lactose is water soluble and also possess good disintegrant activity hence enabling the drug to be released into solution faster whiles microcrystalline cellulose has very good disintegrating properties, but is water insoluble. starch 1500 should have obtained faster dissolution rates as starch 1500 is water soluble and also possess disintegrant activity(but should be included at relatively low concentration 1-5% by weight) and the concentration range of the tablet should be up to 10% this might not have been achieved due to the above factors mentioned (poor powder mix, poor filling of capsule). Thereby the percentage of the diluent used was too large thus affecting the dissolution of the drug, where it may have been faster if a smaller quantity of diluent was used in the formulations instead.
Figure 2 is a graph that shows the % of drug content released vs. time for the commercial paracetamol formulations. It shows that the Panadol Actifast Tablet has the fastest dissolution rate in 15 minutes at 125% and anadin had the percentage of drug in solution is about 121%, and this was followed by the galpharm at 104%. Additionally, all three formulations managed to released over 100% of the drug content into solution which is not expected. This is could be due to inaccuracy in dilution of medium during course of experiment. From results anadin is just as competitive with Panadol actifast Tablet in terms of the speed at which drug is relesed into solution. The price is significantly higher because the Panadol Actifast Tablets contain more excipients like flavours and sweetening agents improving overall appearance of drug and also more excipients that improve mechanical strength of tablet and also performance of tablet as fast relief of pain.Therefore this suggests that a combination of additional excipients present in Panadol Actifast Tablets enhances its dissolution. Anadin which is very close to panadol in the dissolution profile from experiment results. This is because anadin contains croscamellose, which is a superdisintegrant, i.e. a more effective agent aiding disintegration. It has lesser excipients than panadol hence the lower price. From results percentage of Paracetamol galpharm solution is lower. Initial high percentage at 5 minutes can be due to error in carrying out experiment. Furthermore, Paracetamol galpharm has basic excipients that enhance the tablet to dissolve but no extra excipients to improve the dissolution profile, also its cheaper as there are no sweetening agents and flavours.
From graph 1 and graph 2, it can be seen that for each formulation, the % of drug content released increased with time until all the drug had been completely released, after which it steadily decreased. an increased quantity of drug is released into the solution as the formulation dissolves and time increases. But once all the drug has been released from the formulation the % of drug content decreases as there is lesser drug in solution making it more diluted, due to addition of distilled water at each time the sample is withdrawn from the dissolution apparatus.Comparison between the two reveals that tablets dissolve in solution much faster than capsules, as the tablets release their total quantity of drug present into solution quicker time between (40 -45 minutes). However, in order to carry out a comparison between dosage forms, the same excipients need to be present in both the capsule and the tablet formulation, which is not the case here, and therefore the difference observed could be entirely due to the differences in the excipients present, in particular the presence of pregelatinised maize starch which is a disintegrating agent only present in the tablets.
CONCLUSION
The results obtained enable the conclusion that paracetamol capsules containing the diluent lactose undergoes faster dissolution in comparison to formulations containing starch 1500 and microcrystalline cellulose, where microcrystalline cellulose formulations was the slowest dissolution. Additionally, the commercial paracetamol formulations results enable the conclusion that Panadol Actifast Tablets and anadin undergo faster dissolution with higher percentage of drug released compared to that of galpharm hence are more convenient when fast relief of pain is required. Furthermore, it also enables the conclusion that the more expensive the product the better its quality, as it contains more excipients that could potentially aid the dissolution of the formulation, improve mecahanical strength and flavouring and also appearance of product.
Percentage of drug content released into solution was greater than 100 prior to obtaining a mean value, but this would indicate that more drug is being released into solution than there actually is available to be released. Also an explanation, behind this is that perhaps the actual drug content in the formulation is more than that stated or that there is some other excipient in the formulation that absorbs UV light in the same wavelength range as paracetamol, causing interference in the reading thus giving higher values. A possible way to avoid this problem in future would be to use a more reliable method to assess the concentration.
From the practical it is difficult to conclude whether tablets dissolve in solution faster than capsules, as the excipients present in the formulations weren’t entirely the same. In future this practical could be further improved by formulating capsules containing the same ingredients as a tablet and then testing their dissolution to see whether differences in dosage form influence the dissolution rate. Also the entire procedure in conducting experiment is not reliable as in using the syringe to take out the solution this was not taking out the accurate volumes.
REFERENCES
Aulton’s Pharmaceutics: The Design and Manufacture of Medicines. Third Edition.
Edited by Michael E. Aulton.
Scientific principles of dosage form design, Chapter 2: Dissolution and solubility,.
Dosage form design and manufacture, Chapter 31: Tablets and compaction, page 463.