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An investigation into the effects of temperature on the enzyme renin.

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An investigation into the effects of temperature on the enzyme renin. Background An enzyme is a biological catalyst, which is made up from a series of complex proteins and is formed, in a complex three-dimensional shape due to the way the amino acids are folded. A catalyst is a substance that will speed up the rate of control in metabolic reactions without exhausting itself, if an enzyme does not denature they will then generate other molecules. The enzyme renin can be found in the stomach of all mammals, it plays an important role in the body of where it's function is to solidify milk this is important for young mammals. An enzyme has a particular shape this means that only substrate with the same unique shape will be allowed to enter the enzyme active site. This theory is known as lock and key theory. An active site is where the substrate fits into the enzyme and only a small part of the enzyme come into contact with substrates. Enzymes can be temperamental and can be denatured easily if conditions are wrong. ...read more.


Increasing and decreasing the amounts of substances used at varied heat settings showed us that the best results were happening at variables of 10?c so then we decided on the following temps of 30?c, 45?, 55?c, 80?c. Method For this experiment we took a total of 16 test tubes using a 5ml syringe 2mm of milk were placed into each of the tubes, these where then labelled for the relevant temperature to avoid confusion. Four water baths where then pre - heated to temperatures of 30?c, 45?c, 55?c, 80?c. a thermometer was used in each of the baths to ensure that the temperature was monitored at the required level to make the test fair. Once the water baths had reached the required heat setting, four of each temperature samples where added to each of the water baths where they where then left for ten minutes. Once the samples had reached the required heat and removed from the bath's they where then transferred into a test tube rack to keep the sample stabilised. Immediately once removed two drops of the enzyme renin where then added into 3 of the test tubes leaving the forth one to remain with just milk and no renin. ...read more.


As the body's temperature always is around the 37?c with very slight variables, this shows that the most amount of kinetic energy is apparent at this point causing the most collisions between the various molecules. Evauation As can be seen from the experiments, the test was successful in proving the effects of temperatures on the enzyme renin. Although there were some minor factors that could stop the experiment from being fair, A main factor in this was the water baths that where being used for the experiment where getting old and unreliable it was hard for us to maintain a steady temperature. This caused the results to become unsteady. Other factors that could be seen to have a negative effect on the out come were the amount of solution being used. In the preliminary working we found that if too much milk was used it would be difficult to determine weather the milk had started to clot or not, freshness of the milk also could have played a negative role. If the milk was old and began to turn sour this could have been confused for a clot encouraged by the renin. ?? ?? ?? ?? Gareth Ayers Methodology ...read more.

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