• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

An Investigation on the affect of temperature on the rate of reaction.

Extracts from this document...


An Investigation on the affect of temperature on the rate of reaction Aim: The aim of this investigation is to determine how varying temperatures affect the rate of reaction. Hypothesis: I predict that the higher temperature gets the faster the rate of reaction. However, as catalase is an enzyme found in the human body as well, beyond 40oC the enzyme will be denatured. Enzymes are protein molecules that are specific in recognising and binding to specific reactants known as substrates. Enzymes speed their conversion into products. These proteins are responsible for increasing the rates of all of the many thousand of reaction taking place inside cells. A number of external factors affect the activity of enzymes and the rate of these catabolic or anabolic reactions. These factors include variations in the concentration for substrate molecules, enzyme concentration, presence of inhibitors and pH. Catalase is an enzyme found in many things such as peas, potatoes and the liver. It is used for removing Hydrogen Peroxide from the cells. Catalase speeds up the decomposition (catabolic reaction) of Hydrogen Peroxide into water and oxygen. It is able to do this due to the shape of the Hydrogen Peroxide molecule and its own active site. Substrate + enzyme Products + enzyme Hydrogen peroxide + Catalase Water + Oxygen + Catalase 2H2O2 (l) + Catalase 2H2O (l) ...read more.


Set the apparatus accordingly: 2. Place 3 boiled peas in test-tube half-filled with distilled water, in another test tube place 3 unboiled peas in the same way. 3. Place these test tubes in the water bath of the required and set temperature, in this case 30oC, for a minimum of 15 minutes to allow the peas to form an equilibrium with the temperature in the water bath. 4. After the peas have equalised in temperature, they can be removed from the test-tube and the coat can be removed. 5. Crush the peas in the pestle and mortar to increase surface area for the reaction. Then place in the conical flask. 6. Get the stopwatch ready and pour 10cm3 of Hydrogen Peroxide into the conical flask and quickly replace the bung. The stopwatch should immediately be started as soon as the substrate is poured in. 7. Record the volume of gas collected every 30 secs for a total duration of 5 minutes. 8. This method should be repeated 3 times for each temperature. Apparatus: * Conical flask * Pipette * Gas syringe * Thermometer * Water baths * Ice * Peas * Pestle and mortar * Scalpel * Distilled water * Hydrogen Peroxide * Stopwatch Safety: * wash hands after using chemicals * wear goggles at all time during experiment Variables: 1) ...read more.


However, an anomaly can be detected from fig 1.2 which describes the overall trend of rate or reaction. At 200C the total volume of oxygen gas collected decreases from 20.3cm3 at 100C to 18.8cm3. This anomaly could be due the results being obtained from different groups, thus difference in equipment use and procedure may have produced this variation. Moreover, this result could be due to the fact that a controlled electronic water-bath was not used for this particular temperature, thus the temperature was not constantly maintained during the course of the reaction. Moreover, perhaps shaking or stirring the flask around would allow each enzyme and substrate to bind equally or fairly which would lead to more accurate results. To improve the investigation, temperatures below 00C should also have been investigated, as there was oxygen production even at this temperature. Moreover, more specific temperatures should be investigated such as 300C, 320C, 340C, 360C, 380C etc to gain a specific ideal that suits the enzyme and thus causes an increased rate of reaction. Moreover, perhaps shaking or stirring the flask around would allow each enzyme and substrate to bind equally or fairly which would lead to more accurate results. The investigation could also be extended to combine other factors affecting the rate of reaction such as pH, enzyme concentration, presence of inhibitors, or substrate concentration. Ambreen N.Khan 12T6 1 ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. An Investigation Into the Effect of Substrate Concentration On the Rate of Enzyme Activity.

    Test Results from non-immobilised and immobilised catalase enzyme for Significance of Difference (20oC) - Mann Whitney U Test Rank (R1) 1.5 1.5 3.5 3.5 5.5 5.5 immob. 5 5 7 7 8 8 Rank (R2) 7.5 7.5 10 10 10 12 non-immob.

  2. Amylase Investigation

    This is thought to be due to the fact that there are so many substrate molecules competing for the active sites on the enzyme surfaces that they block the sites (Figure 9) and prevent any other substrate molecules from occupying them.

  1. Trypsin. Hypothesis: - I hypothesize that as the temperature increases the rate of enzyme ...

    Antioxidant enzymes like Catalase and superoxide dismutase are located in the periplasmic space, which is the space between the inner and outer membranes of the cell wall. There are numerous enzymes located here that would be toxic if they were found inside the cell.

  2. Catalyse Investigation

    air leaks into the burette when it is inverted with the open end underwater. Clamping the apparatus in place enables me to concentrate on running the experiment and not have to hold anything. 3. 3. The tube must be fixed under the burette, with a funnel directing the air bubbles into the burette.

  1. Catalase investigation

    and tube for refuse Method * The apparatus was set up as is shown in the diagram below. * 2.5g of liver was carefully cut up and weighed. * One pipette was used to drop 5ml of H2O2 into one boiling tube, followed by 5ml of pH 2 buffer solution.

  2. Investigate how concentration of the enzyme catalase in celery tissue alters the rate of ...

    Repeat step 13, 26 and 14 to achieve a 5 cm3 sample of hydrogen peroxide. 41. Repeat steps 16, 18, 19 and 20, recording the results next to the "60% celery extract/catalase concentration" row and the parallel columns. 42.

  1. Factors That Affect the Rate of an Enzyme Reaction.

    The enzyme substrate complex goes through a transition stage where it is neither substrate or product. 3. A complex of the enzyme and the product is produced 4. The product is finally made and the enzyme and product separate Factors that affect the activity of enzymes Factors that affect

  2. An Investigation into the effect of varying enzyme concentration on the rate of an ...

    Bibliography To find my background information I used: * My AQA biology coordinated award book * My notes from class I have to keep the pH and temperatures the same throughout the experiment otherwise the enzymes will denature. Extreme pH level also makes the enzyme denature.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work