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An investigation to find the best temperature for the enzyme Amylase

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Introduction

Science Coursework An investigation to find the best temperature for the enzyme Amylase Aim: The aim for this experiment is to find out the best temperature for the enzyme amylase and at what temperature of water it works the fastest. I plan to collect evidence to prove that the enzyme amylase will work best at the temperature 37�C. I will choose a range of temperatures two below 37�C and two above 37�C so I will be able to determine what temperature it works best at. Prediction: I think that the enzyme amylase will work the best and fastest at 37�. I think this because this is at body temperature so amylase works at its best to digest large insoluble food molecules into smaller simpler and more soluble molecules to be digested. Scientific knowledge: The scientific knowledge I know is that amylase digests insoluble starch molecules into smaller soluble molecules of sugar (glucose). Enzymes are biological catalysts. They speed up the rate of a reaction without themselves being used up. Enzymes are individual proteins which controls a particular reaction or type of reaction. They are specific e.g. amylase always acts on starch. Enzymes can be used over and over again. They can work up to temperatures of 40�C but if the temperature goes above 45�C the enzymes become denatured and cannot work anymore. ...read more.

Middle

Put your bags and coats under the table so no one trips over them and injure them self. Don't squirt people with the pipettes because if it was iodine it would stain their clothes. Don't mess around because you could break some of the schools science equipment. Results and observations: Temperature �C Temperature in minutes 1 2 3 4 5 6 7 8 9 10 18�C 1 2 ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( 28�C 1 2 ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( 37�C 1 2 ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( 56�C 1 2 ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( 78�C 1 2 ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( The results labelled 1and highlighted red in the Temperature �C column are my results and the results labelled 2 and highlighted blue are someone else's results so I can see if mine are accurate enough. ...read more.

Conclusion

One anomaly in this experiment could be that the iodine in the bottle already contained a little starch which spoilt the experiment. The procedure I did I think is very suitable and it doesn't have much defects but they can easily be corrected. You can make sure that the experiment you do can come out with the best results by making sure you have clean iodine, you don't mix up the pipettes, you put only three drops of amylase to the starch, and you start the stop watch/clock when the first drop goes in. You could choose more temperatures for the experiment so you will get more results and see what temperatures it works best at. To make sure the results are accurate you should do the experiment with the different temperatures more than once and then after you can compare it with other peoples to see if your results are correct. The evidence I gathered I think is sufficient enough to support my conclusion because my scientific knowledge tells me lots of things about the enzyme amylase and the optimum temperature at which it works best at. To provide additional relevant information to make this experiments results more accurate you could research a scientist who has done this experiment before and see the results you got and compare it to their results or you could do further work which will make sure you come out with the best results as possible. ...read more.

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