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Analysis on The Effect of Different Temperatures on the activity of an Enzyme.

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[HUMAN BIOLOGY: Practical Assessment] Target: Analysis on The Effect of Different Temperatures on the activity of an Enzyme Background Knowledge Enzymes are proteins and they act as catalysts increasing the rate of chemical reactions and. work best at a special temperature of 37� within the human body. They work only within a small limited target range of pH and by binding to the substance they change. Each enzyme works only on a specific substrate and is denatured by heat greater than 55�C and once they change the substrate, they can then be used again. Substrate is the substance before any form of reaction has taken place. They are substances that are awaiting a chemical reaction to transform them into a new melded product. Products are a post-reaction, i.e. it is a new substance formed after a catalytic reaction like maltase, which chemically breaks maltose down to form glucose, where glucose is the end product and maltase is the catalytic enzyme and maltose is the substrate or carbohydrate. ...read more.


Hence if you heat enzymes above 40C, they start to waver and work less efficient, but if you heat them greater than 70^0C, they form shape so much they can never work again and become denatured, i.e. destroyed. Therefore, from my knowledge above I assume and predict that binding the substrate Hydrogen Peroxide with Catalase , which is a heme containing redox enzyme and is found in high concentrations in a compartment in cells known as the peroxisome, will break down the substrate to produce two end products being 'water' and ' oxygen'. As the quantity of the enzyme is irrelevant then the oxygen end product can be measured by marking in 'ml' to indicate the variations in enzyme activity in various temperature bandwidths. Hence, I predict that at higher temperatures enzyme activity will be decreased eventually to none as opposed to lower temperature minimal enzyme activity and at ideal body temperature enzyme activity will be very active. ...read more.


Results The Short table below shows the results My conclusion Although the data collected is not completely accurate due to the experiment not being completely fool proof as it was not in a secure environment, but from the graph and the table we can see that my hypothesis and/or theory was correct that at low temperatures Enzyme activity is minimal, at high temperatures above 70+ it becomes denatured due to the fact that the enzymes are broken down too quickly before they can recreate and hence destroyed; and at body temperature of 37 degrees, the ideal temperature for the enzyme we can see maximum efficiency. Therefore, when Temperature <37 enzyme activity is minimal or very little but when temperature > that of 48, the enzyme activity creeps towards denature and when temperature = 36-38, we can see that it is ideal temperature for the enzyme to flourish to produce higher amounts of oxygen and breaking down of the substrate is more efficient. I ...read more.

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