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Enzyme Investigation.

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Introduction

Enzyme Investigation Enzymes such as Catalase are protein molecules, which are found in living cells. They are used to speed up specific reactions within the cell. They are all very specific as each enzyme just performs one particular reaction. Catalase is an enzyme found in food such as potato and liver. It is used to remove Hydrogen Peroxide from cells. Catalase speeds up the decomposition of Hydrogen Peroxide into water and oxygen because the shape of its active site matches the shape of the Hydrogen peroxide molecule. This type of reaction where a molecule is broken down into smaller pieces is called a catabolic reaction. This is the reaction equation for the breakdown of hydrogen peroxide into water and oxygen: Hydrogen Peroxide + Catalase = Water + Oxygen. 2H202 2H20 + 02 Aim: The aim of this experiment was to examine the effect of catalase on the breakdown of the substrate hydrogen peroxide. The catalase used was obtained from a piece of liver. An enzyme is a biological catalyst. Catalysts speed up reactions. Most enzymes optimum working temperature is 37 (body temperature), however the optimum working temperature of catalase is 30 . ...read more.

Middle

With more successful collisions with more energy, the amount of oxygen should be produced at a faster rate, which is how the graph has turned out. The graph more or less resembles my predicted graph and my prediction. Looking at the graph I will explain why the set temperatures on the graph are correct or not. 18 C -The line of best fit produced fits in with my prediction and the theories stated on the previous pages. Whilst the optimum temperature of a catalase is higher than that of room temperature (app 20 C), this shows that oxygen has been given off at an acceptable rate at room temperature. There was enough successful collisions and energy to produce oxygen from the reaction, so is not regarded as an anomalous result. 33 C - In my opinion I would regard this scatter as anomalous. This is because it does not tie in with the rate of reaction theory. 33C is near an enzymes optimum temperature and so should produce more oxygen in a shorter space of time, but this is not the case. This could have been due to a recording error or a disruption to the independent variables. ...read more.

Conclusion

Obviously, the experiment could have been improved by repeating the experiment more times to calculate a better average. I think there was sufficient evidence to draw firm conclusions from the results as it agreed with my prediction and proves many of the reaction theories. Many variations of this experiment could back up the evidence I already have. The independent variables could be changed. For instance: the hydrogen peroxide concentration, differently shaped sizes of the liver and surface area. How much enzyme is in 1 gram of liver is questionable. The same weight doesn't truly determine the same amount of enzyme contained in the liver. So if a piece of the liver was collected from the centre of the liver it may contain more or less enzyme than the edge or vice verse. It may even contain the same amount of enzyme all over. The liver was probably kept over a series of days and this would of affected the amount of water in the liver. If the water evaporated the catalase would be in a dehydrated state. This would mean that the weight may stay the same or decrease and the amount of enzyme would also increase or decrease. To overcome this problem, it would be wise to dehydrate all the liver or keep the whole of the liver moist. By Arun Joshi ...read more.

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