• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Enzymes - find the optimum temperature that a protease enzyme works at.

Extracts from this document...

Introduction

Biology Coursework - Enzymes. Planning. Aim - the aim of the experiment is to find the optimum temperature that a protease enzyme works at. To do this I will need to plan a safe experiment that will be a fair test. For the experiment I have chose to use some photographic film, which is covered in silver salts, the silver salts are held on by a protein glue. Silver Salts Protein Glue Transparent Film If the experiment goes according to plan then the protease will digest the protein glue, turning it into amino acids and so the silver salts will fall off. This will then mean that only the transparent film is left and so as soon as I can see through the film I will stop timing. I can do this by altering one of the two variables of the experiment. Safety - To keep the highest standard of safety during this experiment I must follow some simple rules when using the equipment: * When using the test tubes you must also use a test tube holder so there is less chance of the test tubes getting smashed. * Be very careful when using the water bath as it will be at temperatures of up to 800C. * Handle scissors carefully as these are sharp wire cutting scissors. Variables - there are two main key variables in this experiment, by varying one of them I can complete the experiment and find the results that I need. ...read more.

Middle

The graph shows that the optimum temperature for a particular enzyme is 37oC - this is its optimum temperature after this it becomes DENATURED. Enzymes - shape. Every enzyme has a particular shape, which is individual to it, this means that on enzyme can only digest on type of food, for example: - PROTEASE PROTEIN CARBOHYDRASE CARBOHYDRATES LIPASE FATS Lock and Key. The enzyme digests the food molecule in the same way that a key opens a lock, only one enzyme can digest a particular food molecule. Above is a diagram of how an enzyme works, the food molecule on the right is the perfect fit for the enzyme and it would be the only one of its kind so no other food molecule could fit this enzyme and vice versa. The red part of the enzyme is the active site. The active site binds the substrate and then the enzyme digests it. Method- 1. Collect the apparatus that is needed to do the experiment. 2. Cut a strip of photographic film from the same area on the big role of film (the length should be about 40 holes on either side). 3. Cut photographic film up into smaller pieces and thread them with a piece of wire that is longer than a test tube. 4. Using a syringe put 2ml of the enzyme into each of the four test tubes. ...read more.

Conclusion

The curve on the graph is quite reliable but not perfect, it gives a good idea of what the perfect curve would look like. I have one or two anomalous results, I expect these are because I could only check one of the test tubes at a time which means that the salts may have fallen off in the last test tube but I was only getting round to checking the second test tube. This means that the average time for that temperature would be slowed down and so the rate would be lower and this would affect the graph. If I were to do this experiment again I would definitely use more test tubes and I would get more people to check the test tubes more often to give a more accurate result. To extend the enquiry I could do one of two things, I could find the optimum temperature for other enzymes such as carbohydrase and lipase, and I could use the result to see if there were any connections in optimum temperatures between all the enzymes. Another test I could do is to see what the optimum pH for the protease enzyme would be. To extend the experiment that I have already done I could find what I have recorded as the two fastest rate temperatures and do one last experiment. So if the highest two rates were 500C and 600C this would mean I should repeat the experiment at 550C, this should give me a more accurate optimum temperature. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Marked by a teacher

    Find out if enzymes work faster or slower at different temperatures.

    5 star(s)

    This is why we need to use 20cm3. Using over 20oc would give a higher overall time, using under 20oc would give a slower time or possibly a time of zero if there is not enough to obscure the cross at the beginning. We will need to keep the concentration the same for both experiments as doubling the concentration percentage means doubling the amount of enzyme in solution.

  2. An experiment to show how protease enzymes are affected by temperature

    This shows that this temperature is not a very suitable temperature for enzymes to be digested at * At 20�C the reaction had speeded up * At 30�C, the reaction had speeded up dramatically, this is possibly because 30�C is close to body temperature.

  1. A2 coursework- The effects of bile salts on digestion of fat

    I can also then calculate the rate of reaction within a time for the reactions to prove my prediction correct if so. Safety: The experiment should be kept organized and so as safe as possible. There are a number of risks that should be addressed before out taking the experiment.

  2. Liver and its role

    I will have to use same cream at all time, as different creams may have different amount of the fats in them. After collecting apparatus I will use small beaker to pour some of the cream, which then will be used in the actual experiment.

  1. Investigation to show the effect of temperature on the action of protease on photographic ...

    They are specific- Each enzyme controls a specific reaction and will only act on certain substrate. 3. They can be reused- enzymes remain unchanged throughout reactions,so they can be used over and over again. 4. They become deformed by heat-.almost all enzymes become denatured, when the temperature rises above a certain temperature.

  2. Biology Coursework on Enzymes.

    is kept the same in order for it to be a fair test. Problems that were encountered in the Preliminary experiment: After carrying out my first preliminary experiment I encountered several problems. Here are the problems that I encountered and the solution of the problem: The very first problem that

  1. The aim of this experiment is to determine the effects of varying temperatures on ...

    Control Variable: Volume of Hydrogen Peroxide, volume of Catalase solution, concentration of Hydrogen Peroxide, concentration of Catalase. . HYPOTHESIS I predict that the greatest voloume of oxygen will be produced at body temp which is 37 �C as this is the temperature are bodies are and enzymes are designed to work best in this condition.

  2. A photographic company wants to recycle the plastic in exposed (and therefore unusable) photographic ...

    pH is too high or too low for the enzyme, it will become denatured, where the structure of the enzyme is changed so that substrates will no longer be able to fit inside the enzyme and therefore will not be digested.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work