• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Experiment to investigate the effect of temperature on protease enzyme

Extracts from this document...


Paul Haycock Biology Coursework ????????????????????????????????? ??????? ?????????????????????????????? ???????????????????? An enzyme is a biological catalyst. This means that they speed up chemical reactions in the body without changing themselves. Enzymes are protein molecules which are usually denatured (damaged) by temeratures above about 45C. Enzymes work, like all catalysts, by providing a different pathway for the reaction to follow. The new reaction path has a lower activation energy and so the rate of the reaction is much faster than if no enzyme is present. Enzymes catalyse reactions using a lock and key mechanism. The reactants fit exactly into the enzymes active site and when the reaction has taken place the products are relesed from the enzyme, allowing it to catalyse another reaction. In humans enzymes are made in the pancreas, passed into the digestive system and secreted onto food in the stomach. This helps braek down the larger food molecules into smaller ones so that they cross the gut wall and enter the blood. Enzymes are also used in biological washing powders to help cleaning by dissolving stains like grass, blood etc. ...read more.


????????? The equipment I will be using is as follows: Water bath, Protease enzyme, Photographic film, Test tubes, Thin wire, 2 Thermometers and a Syringe. (see diagram below) ?????? To do this investigtion I will set up the equipment as shown on the previous page and continue as follows. First I will set the water bath to the desired temp. Then I will prepare my three test tubes by adding 3cm of protease enzyme to each. Next I will place the the test tubes into the water bath and use the thermometers to tes both the water and enzyme temp. When both of the thermometer readings correspond I will place the three piece's of photographic film into the three test tubes all at the same time so that mistakes in timing are not made. As the photographic film goes into the enzyme another person will start the stopwatch, this is so that the times are not shorter than they should be. Then I will keep checking the experiments to see if the enzyme has finished working. ...read more.


As can be seen by the results the enzyme must vibrate more as it reaches its peak temperature. As it reaches its peak temp. therefore it takes less time to break down the protein as it has more succesful collisions per second. As the temp. rises above the peak the process slows down and eventually comes to a stop as the enzyme denatures. When the protease enzyme is at a very low temperature, it does not vibrate as much or not at all. This means either less or no sucessful collisions per second. Therefore the protein does not get borken down but it does not mean the enzyme has denatured, as my results show. To improve my investigation I could take lots more readings around the peak temp. area to really pin down the actual peak at which the enzyme works. But as I had limited time this would be impractical. I could also look at other aspects of the experiment such as, does the concentration of the enzyme have any effect on the speed of the process. If I was to do more work on this, that is the aspect I would look at. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Investigation to show the effect of temperature on the action of protease on photographic ...

    It would also help me decided upon suitable equipment that could be used for my actual experiment it also gave me an idea of how to judge the end point of the reaction. Theory Enzymes are biological catalysts, made from proteins.

  2. A photographic company wants to recycle the plastic in exposed (and therefore unusable) photographic ...

    These factors are the pH of the environment, the temperature, the concentration and inhibitors. The factor that I will be controlling in this experiment is temperature. Prediction The factors that will affect the rate of reaction of Trypsin are.... pH This can alter the rate of reaction because if the

  1. An experiment to show how protease enzymes are affected by temperature

    Then 2 beakers, 3 boiling tubes, 3 test tubes and a thermometer were set up as shown in the diagram on the previous page. Next using a measuring cylinder, each one of the 3 boiling tubes where filled with 30ml of marvel milk.

  2. All flesh is grass.

    All plants need nitrogen as it is essential to the formation of nucleic acids and protein. However plants can only take up nitrogen in the form of inorganic ions, in the forms of NO3- (nitrate) or NH4+ (ammonia). The organic compounds left in the detritus are converted to inorganic ions


    We could have also used a broader range of temperatures to enable even better results. And repeating this experiment a few more times could help prevent inaccuracies. CONCENTRATION AS A FACTOR Aim: - To investigate how the concentration of a substrate and enzyme effects enzyme activity.

  2. The Effect of Temperature on Protease.

    Only the folded structure forms the catalytic or active site. However this folded structure will generally be held together by non-covalent interactions unlike the covalent bonds that hold the amino acid links together. These consist of interactions such as ionic (salt)

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work