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How does temperature effect respiration?

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Introduction

How does temperature effect respiration? Natalie Onions Joseph Whitaker School To investigate how temperature effects respiration, we would have to use something which contains living enzymes so that the limitations of which temperatures can be investigated are present. If enzymes are exposed to temperatures too far above or below their optimum range, they will denature and the experiment will be unsuccessful. (Nelson's Modular Science book). Equipment: Yeast solution (20gramms for each temperature) Beaker Water Scales Kettle Clamp stand Conical flask Gas syringe Sugar Spatula Measuring cylinder Thermometer Stopwatch Bubble wrap The Gas syringe was the most useful piece of equipment made available to us. A pipe is connected to a bung and placed on top of the conical flask. The other end was then connected to the gas syringe. The yeast will be respiring aerobically and so will be producing Carbon Dioxide gas. If the yeast should, for any reason, start to respire anaerobically, it will still produce carbon dioxide gas. This is the gas that we will be collecting. Any gas produced by the yeast solution in the flask travelled to the syringe and pushed the plunger upwards. This allowed us to take a very accurate reading of the amount of gas produced. ...read more.

Middle

Method: Using the measuring cylinder, measure out 20ml of yeast solution and pour it into the conical flask. Make up a water bath to the temperature that you have chosen. Do this by adding hot water from the kettle to cold water from the tap in the beaker. Measure the temperature with a thermometer. Do not fill the beaker too high or when the conical flask is placed inside, it will overflow. When filled, wrap the beaker with bubble wrap to maintain the temperature. You will need the gas syringe and piping set up ready in the clamp stand as in the diagram above. Add your chosen amount of sugar to the yeast, swill around to begin respiration and immediately place the bung with the piping in the top of the flask. After 30 seconds, take the reading on the gas syringe and take note of this. Repeat this every 30 seconds for 3 minutes, taking note of the reading every 30 seconds. After 3 minutes, remove the bung from the flask and get rid of the flask's contents into the sink. Wash any remaining sugar and solution from the bottom before restarting with another temperature. Also, you must not forget to push the gas syringe plunger back down to the bottom before doing your next part of the experiment. ...read more.

Conclusion

The hardest part of the experiment was keeping the yeast solution moving. This had to be done to make sure that the solution was always respiring aerobically and producing carbon dioxide gas. Aerobic Respiration: Glucose + Oxygen Carbon Dioxide + Water + Energy C6H12O6 + 6O2 6CO2 + 6H2O + 35ATP We found it quite easy to take the measuring on the syringe every 30 seconds as there were 3 of us in our group and so we could give one person the job of telling another when to take a reading and the third could then take note of this reading. With the bubble wrap around the water bath in the beaker, the temperature of the water stayed constant for each part of the experiment. If we were to repeat the experiment, I would like to find a way of taking the temperature of the yeast solution throughout. This wasn't possible for us as the top of the flask had to remain tightly sealed throughout the experiment to prevent the escape of any gas through the top. If we could have taken the temperature of the yeast, we could have performed the experiment at 36 degrees and used that result as a base to compare everything else to. ...read more.

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