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How to use quantity of glucose concentration in body fluid in clinical diagnosis.

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Introduction

Quantitative Ben edict Background Information's of Glucose. Glucose is form family of carbohydrates. This is important in many biological processes. Glucose is starting point for the manufacture of many organic compounds within the body, and it's major fuel for cellular respiration. It's transported in blood plasma. Glucose is so vital that human have evolved a complicated system in maintaining blood glucose concentration at a steady state. If the blood glucose concentration in human is too high (hyperglycaemic) or too low, this is known as diabetics. When this happened kidney wont is functioning as it meant to, which is inabilities to reabsorb all the glucose filtered into its tubules back into the blood. Glucose is extracted in urine, but normally there shouldn't be glucose present in body fluid (urine). When glucose is present in urine it's called glycosuria. This is sign of diabetics. Glucose is mostly known as a hexose, which means 6 in mathematical term, but in biochemist term its n 6, and the most popular formula for glucose is (CH2O)n - using this formula, glucose chemical equation could be worked out. Which is, (CH2O)6 - This means that the 6 numbers outside the bracket is multiplied by each number of element in the bracket. ...read more.

Middle

And the reading are recorded in Optical Density (OD) Key Controlled Variables: In this experiment some things will have to be kept variable. These are * Volume of water which should be 3cm3 * Volume of glucose, which should be 3cm3 , but at different concentration in each test tube. * Drops of Benedict's reagent, which are 4. Keeping all these will give more chance of getting a reliable result. Equipment * 6 clean Test Tubes * 1 Test Rack * 6-7 Pipette * Water Bath * Spectrophotometer * Centrifuge * Centrifuge Tubes * Cuvette Ingredient * 5% concentration of glucose * Distilled Water * Benedict's Reagent. Procedures * Collect 6 clean test tubes (sizes doesn't matter) * Collect I test tube rack. * Position the 6 test tubes in test tube rack * Labelle the test tubes letter A-F (alphabetically) * Pipette 6cm3 of 5% glucose concentration in test tube a. * Using clean pipette, pipette 3cm3 of distilled water in test tube B to F * Using clean pipette, take 3cm3 of 5% glucose concentration from test tube A, then add to test tube B to make the concentration of glucose in test tube B 2.5% * Using clean pipette, pipette 3cm3 of solution in test tube B to test ...read more.

Conclusion

Evaluation. The in investigation on glucose, and using quantitative benedict's test to observe the presence of glucose in solution was quite understandable also the procedure used, but unfortunately the result was no where being accurate. This is because where the reading meant to be high, because the concentration is wasn't. The inaccurate results are understood to be anomalous result. Thinking of what might have make the result inaccurate might have something to do with the procedure used. To make the result more reliable, it would be recommendable that the method should be repeated with a lot of care. As aimed is concerned, which is using this procedure in clinical diagnosis, this won't be possible, because unreliable the result is. But to make it reliable a very considered, clean, care, and imaginative procedure could be used to make a firm conclusion on how to use the quantitative test for clinical diagnosis, which could give a reliable result. Medically these will discovered how high blood sugar concentration patient have, which is by examining his or her fluid (urine). Other way to test for blood sugar level is by using a method called glucose tolerance test. This when doctor ask patient to swallows a sugar solution, then the doctor measure the blood glucose concentration at interval. Olashubomi Betts Biology Quantitative Benedict Test. 07/11/03 ...read more.

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