• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

How to use quantity of glucose concentration in body fluid in clinical diagnosis.

Extracts from this document...

Introduction

Quantitative Ben edict Background Information's of Glucose. Glucose is form family of carbohydrates. This is important in many biological processes. Glucose is starting point for the manufacture of many organic compounds within the body, and it's major fuel for cellular respiration. It's transported in blood plasma. Glucose is so vital that human have evolved a complicated system in maintaining blood glucose concentration at a steady state. If the blood glucose concentration in human is too high (hyperglycaemic) or too low, this is known as diabetics. When this happened kidney wont is functioning as it meant to, which is inabilities to reabsorb all the glucose filtered into its tubules back into the blood. Glucose is extracted in urine, but normally there shouldn't be glucose present in body fluid (urine). When glucose is present in urine it's called glycosuria. This is sign of diabetics. Glucose is mostly known as a hexose, which means 6 in mathematical term, but in biochemist term its n 6, and the most popular formula for glucose is (CH2O)n - using this formula, glucose chemical equation could be worked out. Which is, (CH2O)6 - This means that the 6 numbers outside the bracket is multiplied by each number of element in the bracket. ...read more.

Middle

And the reading are recorded in Optical Density (OD) Key Controlled Variables: In this experiment some things will have to be kept variable. These are * Volume of water which should be 3cm3 * Volume of glucose, which should be 3cm3 , but at different concentration in each test tube. * Drops of Benedict's reagent, which are 4. Keeping all these will give more chance of getting a reliable result. Equipment * 6 clean Test Tubes * 1 Test Rack * 6-7 Pipette * Water Bath * Spectrophotometer * Centrifuge * Centrifuge Tubes * Cuvette Ingredient * 5% concentration of glucose * Distilled Water * Benedict's Reagent. Procedures * Collect 6 clean test tubes (sizes doesn't matter) * Collect I test tube rack. * Position the 6 test tubes in test tube rack * Labelle the test tubes letter A-F (alphabetically) * Pipette 6cm3 of 5% glucose concentration in test tube a. * Using clean pipette, pipette 3cm3 of distilled water in test tube B to F * Using clean pipette, take 3cm3 of 5% glucose concentration from test tube A, then add to test tube B to make the concentration of glucose in test tube B 2.5% * Using clean pipette, pipette 3cm3 of solution in test tube B to test ...read more.

Conclusion

Evaluation. The in investigation on glucose, and using quantitative benedict's test to observe the presence of glucose in solution was quite understandable also the procedure used, but unfortunately the result was no where being accurate. This is because where the reading meant to be high, because the concentration is wasn't. The inaccurate results are understood to be anomalous result. Thinking of what might have make the result inaccurate might have something to do with the procedure used. To make the result more reliable, it would be recommendable that the method should be repeated with a lot of care. As aimed is concerned, which is using this procedure in clinical diagnosis, this won't be possible, because unreliable the result is. But to make it reliable a very considered, clean, care, and imaginative procedure could be used to make a firm conclusion on how to use the quantitative test for clinical diagnosis, which could give a reliable result. Medically these will discovered how high blood sugar concentration patient have, which is by examining his or her fluid (urine). Other way to test for blood sugar level is by using a method called glucose tolerance test. This when doctor ask patient to swallows a sugar solution, then the doctor measure the blood glucose concentration at interval. Olashubomi Betts Biology Quantitative Benedict Test. 07/11/03 ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Exchange, Transport & Reproduction section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Exchange, Transport & Reproduction essays

  1. Marked by a teacher

    Human Reproductive System

    4 star(s)

    * The brain continues to enlarge, the ossification continues and the body system continues to develop. * The foetus begins to move unobserved by the mother. * Early fingernails start to develop. * The external genitalia are still not distinguishable as male of female.

  2. Peer reviewed

    "An investigation into the Respiration of Carbohydrate Substrates by Yeast."

    5 star(s)

    is being standardised by both the experiments being carried out in aerobic conditions. This is due to the fact that there is a good oxygen supply whilst the yeast is preheated and during gas collection, when the system is air tight, it is not left long enough for it to use up the oxygen and respire anaerobically.

  1. Investigating the stomata density in Hedera up a wall?

    8 from the top, then using the Mann-Whitney U test to show if my hypothesis is right or wrong. I did a preliminary test before my main investigation to the above method which I found worded well and give me relievable results that followed the information that I had found

  2. Rate of Respiration

    and I will use 2 controls per each experiment (test tubes containing separate mixtures of substrate and yeast). However in my plan I stated I will conduct 15 experiments per substrate however this will be too time consuming therefore I will pool my data.

  1. The Endocrine System

    by an autoimmune attack; * an inherited mutation in the ACTH receptor on adrenal cells. The essential role of the adrenal hormones means that a deficiency can be life-threatening. Fortunately, replacement therapy with glucocorticoids and mineralocorticoids can permit a normal life.

  2. The formation of urine.

    Hypertension is a major health problem, especially because it has no symptoms. Many people have hypertension without knowing it. Hypertension is more common in men than women and in people over the age of 65 than in younger persons. Hypertension is serious because people with the condition have a higher

  1. Fundamental human anatomy and physiology.

    the teeth and covering it with saliva, which contains enzymes that starts the digestion process. * The food is turned into a partially digested mass known as a bolus. * Swallowing: when the food is ready to travel to the stomach, the tongue pushes it to the back of the mouth.

  2. Blood System Assignemnt

    Oxygenated blood leaves the left ventricle by Ascending aorta, which takes blood to the body via its system of arteries, arterioles, and capillaries. Major arteries leading from the heart (via the ascending aorta) include the brachiocephalic artery, the left common carotid artery, and the left subclavian artery These are just a few of the main arteries of the body.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work